Literature DB >> 6314259

Construction of infectious potato spindle tuber viroid cDNA clones.

D E Cress, M C Kiefer, R A Owens.   

Abstract

Contiguous restriction fragments from two cloned partial-length potato spindle tuber viroid (PSTV) cDNAs were used to construct recombinant DNAs containing full-length monomeric and dimeric PSTV cDNA. When five different PSTV cDNA plasmids and RNA isolated from E. coli cells harboring these plasmids were tested for infectivity on tomato, plasmid DNAs containing PSTV cDNA dimers were infectious. RNA transcripts containing the sequence of PSTV from these plasmids were also infectious. The sequences of the viroid progeny and the cloned DNA were identical. In vitro mutagenesis of infectious PSTV cDNAs will allow systematic investigation of the role of specific sequences in viroid replication and pathogenesis.

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Year:  1983        PMID： 6314259      PMCID： PMC326416          DOI： 10.1093/nar/11.19.6821

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  33 in total

1.  Citrus exocortis viroid: nucleotide sequence and secondary structure of an Australian isolate.

Authors:  J E Visvader; A R Gould; G E Bruening; R H Symons
Journal:  FEBS Lett       Date:  1982-01-25       Impact factor: 4.124

2.  Longer-than-unit-length viroid minus strands are present in RNA from infected plants.

Authors:  A D Branch; H D Robertson; E Dickson
Journal:  Proc Natl Acad Sci U S A       Date:  1981-10       Impact factor: 11.205

3.  RNA sequences complementary to citrus exocortis viroid in nucleic acid preparations from infected Gynura aurantiaca.

Authors:  L K Grill; J S Semancik
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

4.  Sensitive and rapid diagnosis of potato spindle tuber viroid disease by nucleic Acid hybridization.

Authors:  R A Owens; T O Diener
Journal:  Science       Date:  1981-08-07       Impact factor: 47.728

5.  In vivo recombination of cauliflower mosaic virus DNA.

Authors:  G Lebeurier; L Hirth; B Hohn; T Hohn
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

6.  RNA intermediates in potato spindle tuber viroid replication.

Authors:  R A Owens; T O Diener
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

7.  Direct transfer of cloned genes from bacteria to mammalian cells.

Authors:  W Schaffner
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

8.  Avocado sunblotch viroid: primary sequence and proposed secondary structure.

Authors:  R H Symons
Journal:  Nucleic Acids Res       Date:  1981-12-11       Impact factor: 16.971

9.  Nuclear DNA from uninfected or potato spindle tuber viroid-infected tomato plants contains no detectable sequences complementary to cloned double-stranded viroid cDNA.

Authors:  A Hadidi; D E Cress; T O Diener
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

10.  Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA.

Authors:  P van Wezenbeek; P Vos; J van Boom; A van Kammen
Journal:  Nucleic Acids Res       Date:  1982-12-20       Impact factor: 16.971
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  36 in total

Review 1.  The making of infectious viral RNA: No size limit in sight.

Authors:  M M Lai
Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-09       Impact factor: 11.205

2.  Identification of multiple structural domains regulating viroid pathogenicity.

Authors:  T Sano; T Candresse; R W Hammond; T O Diener; R A Owens
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-01       Impact factor: 11.205

3.  Mutational analysis of potato spindle tuber viroid reveals complex relationships between structure and infectivity.

Authors:  R W Hammond; R A Owens
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

Review 4.  Origin and evolution of viroids and viroid-like satellite RNAs.

Authors:  T O Diener
Journal:  Virus Genes       Date:  1995       Impact factor: 2.332

5.  Use of intramolecular chimeras to map molecular determinants of symptom severity of potato spindle tuber viroid (PSTVd).

Authors:  A Góra; T Candresse; W Zagórski
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

6.  An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.

Authors:  H Puchta; H L Sänger
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

7.  Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus.

Authors:  B Dasmahapatra; R Dasgupta; K Saunders; B Selling; T Gallagher; P Kaesberg
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

8.  Viroid processing: a model involving the central conserved region and hairpin I.

Authors:  T O Diener
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

9.  Production of infectious RNA transcripts from Sindbis virus cDNA clones: mapping of lethal mutations, rescue of a temperature-sensitive marker, and in vitro mutagenesis to generate defined mutants.

Authors:  C M Rice; R Levis; J H Strauss; H V Huang
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

10.  Processing of RNAs of the family Avsunviroidae in Chlamydomonas reinhardtii chloroplasts.

Authors:  Diego Molina-Serrano; Loreto Suay; María L Salvador; Ricardo Flores; José-Antonio Daròs
Journal:  J Virol       Date:  2007-02-07       Impact factor: 5.103
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