| Literature DB >> 6272391 |
Abstract
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.Entities:
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Year: 1981 PMID: 6272391 DOI: 10.1126/science.6272391
Source DB: PubMed Journal: Science ISSN: 0036-8075 Impact factor: 47.728