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Literature DB >> 6365703

Stability of the hybrid plasmid pIM138 and its curing by some eliminating agents.

H Braná, O Benada, O Navrátil, K Cejka, J Hubácek.

Abstract

Hybrid plasmid pIM138 was constructed by insertion of a chromosomal fragment with the threonine operon from Escherichia coli into the pBR322 vector. Molar mass of pIM138 was 2.8 Mg/mol. Heteroduplexes between pBR322 vector and pIM138 hybrid DNA molecules were prepared. The hybrid plasmid shows a high stability against the curing effect of rifampicin and clorobiocin in E. coli SK1590 thr host.

Entities: Chemical Species

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Year: 1983 PMID： 6365703 DOI： 10.1007/bf02879679

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

18 in total

1. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.

Authors: T Eckhardt
Journal: Plasmid Date: 1978-09 Impact factor: 3.466

2. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors: M Dagert; S D Ehrlich
Journal: Gene Date: 1979-05 Impact factor: 3.688

3. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

Authors: J G Sutcliffe
Journal: Nucleic Acids Res Date: 1978-08 Impact factor: 16.971

4. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

Authors: P A Sharp; M T Hsu; E Otsubo; N Davidson
Journal: J Mol Biol Date: 1972-11-14 Impact factor: 5.469

5. Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.

Authors: P Cossart; M Katinka; M Yaniv; I Saint Girons; G N Cohen
Journal: Mol Gen Genet Date: 1979-08

6. Trans-dominant mutation affecting restriction and modification in Escherichia coli K-12.

Authors: J Hubácek; V G Kossykh
Journal: Mol Gen Genet Date: 1982

7. [Cloning of threonine operon genes in Escherichia coli cells].

Authors: Iu I Kozlov; L P Kochetova; V A Livshits; S V Mashko; V N Moshentseva
Journal: Genetika Date: 1980

8. A general method for the purification of restriction enzymes.

Authors: P J Greene; H L Heyneker; F Bolivar; R L Rodriguez; M C Betlach; A A Covarrubias; K Backman; D J Russel; R Tait; H W Boyer
Journal: Nucleic Acids Res Date: 1978-07 Impact factor: 16.971

9. Regulation of the threonine operon: tandem threonine and isoleucine codons in the control region and translational control of transcription termination.

Authors: J F Gardner
Journal: Proc Natl Acad Sci U S A Date: 1979-04 Impact factor: 11.205

Stability of the hybrid plasmid pIM138 and its curing by some eliminating agents.

1. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.

2. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

3. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

4. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

5. Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.

6. Trans-dominant mutation affecting restriction and modification in Escherichia coli K-12.

7. [Cloning of threonine operon genes in Escherichia coli cells].

8. A general method for the purification of restriction enzymes.

9. Regulation of the threonine operon: tandem threonine and isoleucine codons in the control region and translational control of transcription termination.

10. Entrapment of a bacterial plasmid in phospholipid vesicles: potential for gene transfer.

1. Plasmid pIMI38--the pBR322 derivative with increased stability in E. coli cells.

2. Stabilization of a miniderivative of the broad-host-range IncW plasmid pSa by insertion of plasmid R1 parB region.

3. Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria.

4. Production of threonine by Brevibacterium flavum containing threonine biosynthesis genes from Escherichia coli.

5. Curing of plasmid pXO1 from Bacillus anthracis using plasmid incompatibility.

6. Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit.