| Literature DB >> 673857 |
P J Greene, H L Heyneker, F Bolivar, R L Rodriguez, M C Betlach, A A Covarrubias, K Backman, D J Russel, R Tait, H W Boyer.
Abstract
An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.Entities:
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Year: 1978 PMID: 673857 PMCID: PMC342170 DOI: 10.1093/nar/5.7.2373
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971