Literature DB >> 8262444

Production of threonine by Brevibacterium flavum containing threonine biosynthesis genes from Escherichia coli.

M Pátek1, J Hochmannová, J Nesvera.   

Abstract

Genes of the threonine operon of Escherichia coli were used for the construction of a Brevibacterium flavum strain excreting threonine. Using the shuttle vector pCEM300 and a newly constructed shuttle vector pEC71 (7.1 kb, Kmr/Nmr), various plasmids carrying E. coli thr genes were prepared. Mutants resistant to the threonine analog 2-amino-3-hydroxyvaleric acid (AHV) were isolated after the ethyl methanesulfonate treatment of B. flavum carrying these recombinant plasmids. A mutant of B. flavum CCM 351 carrying the cloned genes thrA and thrB accumulated 12 g/L of threonine after 48 h of cultivation.

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Year:  1993        PMID： 8262444     DOI： 10.1007/bf02898754

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  15 in total

1.  A quantitative evaluation of the mutagenic effect of ethylmethanesulfonate in Brevibacterium ammoniagenes and qualitative character of the mutants obtained.

Authors:  H Honzová; B Javůrková; J Skoda; J Dyr
Journal:  Folia Microbiol (Praha)       Date:  1968       Impact factor: 2.099

2.  New and versatile cloning vectors with kanamycin-resistance marker.

Authors:  R D Pridmore
Journal:  Gene       Date:  1987       Impact factor: 3.688

3.  Nucleotide sequence of the homoserine dehydrogenase (thr A) gene of Brevibacterium lactofermentum.

Authors:  L M Mateos; G del Real; A Aguilar; J F Martín
Journal:  Nucleic Acids Res       Date:  1987-12-23       Impact factor: 16.971

4.  Operator-constitutive mutants in the threonine operon of Escherichia coli K-12.

Authors:  I Saint-Girons; D Margarita
Journal:  J Bacteriol       Date:  1975-12       Impact factor: 3.490

5.  Cloning and expression in Escherichia coli of the homoserine kinase (thrB) gene from Brevibacterium lactofermentum.

Authors:  L M Mateos; G del Real; A Aguilar; J F Martín
Journal:  Mol Gen Genet       Date:  1987-03

6.  High efficiency electroporation of intact Corynebacterium glutamicum cells.

Authors:  W Liebl; A Bayerl; B Schein; U Stillner; K H Schleifer
Journal:  FEMS Microbiol Lett       Date:  1989-12       Impact factor: 2.742

7.  [Development of the vector-host system in Corynebacterium. Cloning and expression of homoserine dehydrogenase and homoserine kinase genes in Corynebacterium cells].

Authors:  A L Okorokov; N O Bukanov; O Iu Beskrovnaia; E B Voroshilova; M M Gusiatiner; V G Gritsenko; N K Iankovskiĭ; V G Debabov
Journal:  Genetika       Date:  1990-04

8.  Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria.

Authors:  J Nesvera; M Pátek; J Hochmannová; P Pinkas
Journal:  Folia Microbiol (Praha)       Date:  1990       Impact factor: 2.099

9.  Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom-thrB operon.

Authors:  O P Peoples; W Liebl; M Bodis; P J Maeng; M T Follettie; J A Archer; A J Sinskey
Journal:  Mol Microbiol       Date:  1988-01       Impact factor: 3.501

10.  A C-terminal deletion in Corynebacterium glutamicum homoserine dehydrogenase abolishes allosteric inhibition by L-threonine.

Authors:  J A Archer; D E Solow-Cordero; A J Sinskey
Journal:  Gene       Date:  1991-10-30       Impact factor: 3.688
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  2 in total

1.  Stable Expression of hom-1-thrB in Corynebacterium glutamicum and Its Effect on the Carbon Flux to Threonine and Related Amino Acids.

Authors:  D J Reinscheid; W Kronemeyer; L Eggeling; B J Eikmanns; H Sahm
Journal:  Appl Environ Microbiol       Date:  1994-01       Impact factor: 4.792

2.  Feedback-resistant acetohydroxy acid synthase increases valine production in Corynebacterium glutamicum.

Authors:  Veronika Elisáková; Miroslav Pátek; Jirí Holátko; Jan Nesvera; Damien Leyval; Jean-Louis Goergen; Stéphane Delaunay
Journal:  Appl Environ Microbiol       Date:  2005-01       Impact factor: 4.792
  2 in total

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