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Literature DB >> 390305

Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.

P Cossart, M Katinka, M Yaniv, I Saint Girons, G N Cohen.

Abstract

In vitro recombination techniques were used to clone the Escherichia coli thrA and thrB structural genes in the plasmid vector pBR322. The chimeric plasmid was analyzed and characterized genetically, by restriction mapping and DNA sequencing. The limited expression of the threonine biosynthetic enzymes in the strain carrying the recombinant plasmid is discussed.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1979 PMID： 390305 DOI： 10.1007/bf00267853

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

35 in total

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

2. The primary structure of Escherichia coli K12 aspartokinase I-homoserine dehydrogenase I. Distribution of the methioninyl residues and of the cysteinyl and tryptophanyl tryptic peptides.

Authors: L Sibilli; P Cossart; M A Chalvignac; P A Briley; J M Costrejean; G Le Bras; G N Cohen
Journal: Biochimie Date: 1977 Impact factor: 4.079

3. Large scale purification of a bacterial gene.

Authors: P Tiollais; A Rambach; H Buc
Journal: FEBS Lett Date: 1974-11-01 Impact factor: 4.124

4. Revised structure of aspartokinase I-homoserine dehydrogenase I of Escherichia coli K12. Evidence for four identical subunits.

Authors: F Falcoz-Kelly; J Janin; J C Saari; M Véron; P Truffa-Bachi; G N Cohen
Journal: Eur J Biochem Date: 1972-08-04

10. Construction of hybrid plasmids containing the lysA gene of Escherichia coli: studies of expression in Escherichia coli and Saccharomyces cerevisiae.

Authors: J Chenais; C Richaud; J Ronceray; H Cherest; Y Surdin-Kerjan; J C Patte
Journal: Mol Gen Genet Date: 1981

Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

2. The primary structure of Escherichia coli K12 aspartokinase I-homoserine dehydrogenase I. Distribution of the methioninyl residues and of the cysteinyl and tryptophanyl tryptic peptides.

3. Large scale purification of a bacterial gene.

4. Revised structure of aspartokinase I-homoserine dehydrogenase I of Escherichia coli K12. Evidence for four identical subunits.

5. Secondary-site attachment of coliphage lambda near the thr operon.

6. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

7. Homoserine kinase from Escherichia coli K12.

8. Operator-promoter functions in the threonine operon of Escherichia coli.

9. Threonine locus of Escherichia coli K-12: genetic structure and evidence for an operon.

10. Construction of phi80 dhis carrying Salmonella typhimurium histidine operon mutations.

Review 1. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

2. Construction and physical mapping of plasmids containing the metJBLF gene cluster of E. coli K12.

Review 3. Linkage map of Escherichia coli K-12, edition 7.

4. Localization of the metJBLF gene cluster of Escherichia coli in lambda met transducing phage.

5. DNA sequence change of a deletion mutation abolishing attenuation control of the threonine operon of E. coli K12.

6. Evidence for an internal promoter in the Escherichia coli threonine operon.

7. Nucleotide sequence of the thrA gene of Escherichia coli.

8. Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions.

9. Stability of the hybrid plasmid pIM138 and its curing by some eliminating agents.

10. Construction of hybrid plasmids containing the lysA gene of Escherichia coli: studies of expression in Escherichia coli and Saccharomyces cerevisiae.