Literature DB >> 383576

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

M Dagert, S D Ehrlich.   

Abstract

Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment. With 24-h-old competent cells we obtained routinely 2 . 10(7) transformants per microgram of pBR322 DNA, and transformed over 20% of viable cells.

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Year:  1979        PMID： 383576     DOI： 10.1016/0378-1119(79)90082-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  622 in total

1.  A comparative study of human muscle and brain creatine kinases expressed in Escherichia coli.

Authors:  L H Chen; C B White; P C Babbitt; M J McLeish; G L Kenyon
Journal:  J Protein Chem       Date:  2000-01

2.  Molecular characterization of the two genes SNQ and SFA that confer hyperresistance to 4-nitroquinoline-N-oxide and formaldehyde in Saccharomyces cerevisiae.

Authors:  P Gömpel-Klein; M Mack; M Brendel
Journal:  Curr Genet       Date:  1989-08       Impact factor: 3.886

3.  Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli.

Authors:  M E Barros; D E Rawlings; D R Woods
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490

4.  Expression of genes from the marine bacterium Alteromonas haloplanktis 214 in Escherichia coli K-12.

Authors:  R A MacLeod; R G Hadley; A A Szalay; B Vink; P R MacLeod
Journal:  Arch Microbiol       Date:  1985-08       Impact factor: 2.552

5.  Molecular cloning of SNM1, a yeast gene responsible for a specific step in the repair of cross-linked DNA.

Authors:  E Haase; D Riehl; M Mack; M Brendel
Journal:  Mol Gen Genet       Date:  1989-07

6.  Expression of Treponema pallidum antigens in Escherichia coli K-12.

Authors:  L V Stamm; J D Folds; P J Bassford
Journal:  Infect Immun       Date:  1982-06       Impact factor: 3.441

7.  Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

Authors:  D Shortle; P Grisafi; S J Benkovic; D Botstein
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

8.  Expression of the pilin gene from Bacteroides nodosus in Escherichia coli.

Authors:  T C Elleman; P A Hoyne; D L Emery; D J Stewart; B L Clark
Journal:  Infect Immun       Date:  1986-01       Impact factor: 3.441

9.  Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters.

Authors:  B Lund; F P Lindberg; M Båga; S Normark
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

10.  Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation.

Authors:  M L Stahl; E Ferrari
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490
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