Literature DB >> 358147

pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

J G Sutcliffe.   

Abstract

I have derived a complete restriction map of pBR322 from the total nucleotide sequence of the plasmid. Most of the restriction sites also have been demonstrated empirically. The exact sizes of all restriction fragments and the relative positions of the cuts are presented. These fragments can serve as accurate DNA size markers from small pieces up to the 4362 base pair length of pBR322. Inserts cloned in this vector may be characterized easily using this data.

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Year:  1978        PMID： 358147      PMCID： PMC342202          DOI： 10.1093/nar/5.8.2721

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12.

Authors:  M G Marinus; N R Morris
Journal:  Mutat Res       Date:  1975-04       Impact factor: 2.433

2.  Nucleotide sequence of bacteriophage phi X174 DNA.

Authors:  F Sanger; G M Air; B G Barrell; N L Brown; A R Coulson; C A Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal:  Nature       Date:  1977-02-24       Impact factor: 49.962

3.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  Detection of 5-methylcytosine in DNA sequences.

Authors:  H Ohmori; J I Tomizawa; A M Maxam
Journal:  Nucleic Acids Res       Date:  1978-05       Impact factor: 16.971

5.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

6.  The cleavage site of the restriction endonuclease Ava II.

Authors:  J G Sutcliffe; G M Church
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

7.  A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum.

Authors:  D J McConnell; D G Searcy; J G Sutcliffe
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971

8.  Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.

Authors:  V Hershfield; H W Boyer; C Yanofsky; M A Lovett; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205
  8 in total
  301 in total

1.  Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.

Authors:  E O Long; I B Dawid
Journal:  Nucleic Acids Res       Date:  1979-09-11       Impact factor: 16.971

2.  Deletion hot spots in chimeric Escherichia coli plasmids.

Authors:  B Michel; E D'Alençon; S D Ehrlich
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

3.  Convenience of plasmid R6K higher-copy-number deletion derivative for cloning of larger DNA fragments.

Authors:  J Hochmannová; J Nesvera; J Stokrová
Journal:  Folia Microbiol (Praha)       Date:  1987       Impact factor: 2.099

4.  Construction of a yeast actin gene intron deletion mutant that is defective in splicing and leads to the accumulation of precursor RNA in transformed yeast cells.

Authors:  D Gallwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

5.  Sealing of gaps in duplex DNA by T4 DNA ligase.

Authors:  S V Nilsson; G Magnusson
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

6.  Double-stranded cleavage of pBR322 by a diiron complex via a "hydrolytic" mechanism.

Authors:  L M Schnaith; R S Hanson; L Que
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

7.  Cloning and sequencing of a Moraxella bovis pilin gene.

Authors:  C F Marrs; G Schoolnik; J M Koomey; J Hardy; J Rothbard; S Falkow
Journal:  J Bacteriol       Date:  1985-07       Impact factor: 3.490

8.  Strains of Escherichia coli carrying the structural gene for histidyl-tRNA synthetase on a high copy-number plasmid.

Authors:  S J Eisenbeis; J Parker
Journal:  Mol Gen Genet       Date:  1981

9.  Polymorphisms of mitochondrial DNAs in Norway rats (Rattus norvegicus): cleavage site variations and length polymorphism of restriction fragments.

Authors:  J I Hayashi; Y Tagashira; K Moriwaki; T H Yosida
Journal:  Mol Gen Genet       Date:  1981

10.  Stimulation of recombination between homologous sequences on plasmid DNA and chromosomal DNA in Escherichia coli by N-acetoxy-2-acetylaminofluorene.

Authors:  C Luisi-DeLuca; R D Porter; W D Taylor
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205
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