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Literature DB >> 6273815

Isolation and characterization of cloned rat DNA fragment carrying tRNA genes.

M Lasser-Weiss, N Bawnik, A Rosen, S Sarid, V Daniel.

Abstract

A rat genomic library was screened for tRNA genes with an unfractionated rat liver tRNA probe. About 70 clones containing tRNA genes were detected per rat genome. The organization of tRNA genes in five clones was analyzed by restriction endonuclease digestion, RNA-DNA hybridization and in vitro transcription with nuclear extracts from Xenopus oocytes. Evidence is presented suggesting that tRNA genes are distributed in the rat genome in small clusters spanning 1 to 2 kb and interspersed with large regions (minimum 8 to 20 kb) of non tRNA-coding DNA. The tRNA gene clusters were found to contain the sequences for a variety of tRNA species. Genes for a single isoacceptor, were found in more than one clone. The detailed study of one clone shows the repetition of a cluster of four tRNA sequences at a distance of about 8 kb. The arrangement of tRNA genes in rat appears to follow the irregular pattern of tRNA gene organization previously reported in Drosophila and Xenopus.

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Year: 1981 PMID： 6273815 PMCID： PMC327577 DOI： 10.1093/nar/9.22.5965

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

Isolation and characterization of cloned rat DNA fragment carrying tRNA genes.

1. Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.

2. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae.

3. The isolation of yeast tyrosine and tryptophan transfer ribonucleic acids.

4. Comparative analysis of human chromosomal segments bearing nonallelic dispersed tRNAimet genes.

5. Mammalian tRNA genes: nucleotide sequence of rat genes for tRNAAsp, tRNAGly and tRNAGlu.

6. Evolution of a D. melanogaster glutamate tRNA gene cluster.

7. Analysis of a drosophila tRNA gene cluster.

8. Mapping of transcription units in the bacteriophage T4 tRNA gene cluster.

9. Two drosophila melanogaster tRNAGly genes are contained in a direct duplication at chromosomal locus 56F.

10. Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.

1. A human tRNAGlu gene of high transcriptional activity.

2. Genes and pseudogenes in a reiterated rat tRNA gene cluster.

3. Nucleotide sequence of a segment of human DNA containing the three tRNA genes.

4. Isolation and nucleotide sequence of a plant tRNA gene: petunia asparagine tRNA.

5. Molecular cloning, sequence analysis and in vitro expression of a rat tRNA gene cluster.

6. Isolation and characterization of genomic mouse DNA clones containing sequences homologous to tRNAs and 5S rRNA.