Literature DB >> 6296772

Nucleotide sequence of a segment of human DNA containing the three tRNA genes.

K L Roy, H Cooke, R Buckland.   

Abstract

A 1.65 kb segment of DNA from a human-lambda recombinant, selected for human tRNA genes, was subcloned in the plasmid pAT 153 for sequence determination. Three human tRNA genes were found; one, a tRNAlys gene which appears to specify a tRNA identical in sequence to the previously described tRNA3lys from rat liver (AAA,G); another a tRNAgln gene, the product of which would be expected to recognize only the codon CAG; and a third which would direct synthesis of a tRNAleu specific for CUA and G. Intervening sequences are not found in any of these genes. The three tRNA genes are separated by about 0.5 kb segments of DNA containing no apparent informational sequences. Examination of flanking sequences shows some similarities at the 5'-ends of the three genes, but less similarity to 5' flanking sequences of tRNA genes in other eucaryotes. All three tRNA genes direct synthesis of appropriate sized products in a HeLa cell lysate in vitro transcription system.

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Year:  1982        PMID： 6296772      PMCID： PMC327006          DOI： 10.1093/nar/10.22.7313

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  32 in total

1.  Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.

Authors:  M Smith; D W Leung; S Gillam; C R Astell; D L Montgomery; B D Hall
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Base-specific reactions useful for DNA sequencing: methylene blue--sensitized photooxidation of guanine and osmium tetraoxide modification of thymine.

Authors:  T Friedmann; D M Brown
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

4.  Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.

Authors:  R A Laskey; A D Mills
Journal:  FEBS Lett       Date:  1977-10-15       Impact factor: 4.124

5.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

6.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

7.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

8.  The use of thin acrylamide gels for DNA sequencing.

Authors:  F Sanger; A R Coulson
Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

9.  Delimitation of a promoter for RNA polymerase III by means of a functional test.

Authors:  J L Telford; A Kressmann; R A Koski; R Grosschedl; F Müller; S G Clarkson; M L Birnstiel
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

10.  The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.

Authors:  R M Lawn; E F Fritsch; R C Parker; G Blake; T Maniatis
Journal:  Cell       Date:  1978-12       Impact factor: 41.582
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  24 in total

1.  Compilation of tRNA sequences and sequences of tRNA genes.

Authors:  M Sprinzl; N Dank; S Nock; A Schön
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

2.  Binding of human glutaminyl-tRNA synthetase to a specific site of its mRNA.

Authors:  B Schray; R Knippers
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

3.  Nonsense and missense translational suppression in plant cells mediated by tRNA(Lys).

Authors:  Z Chen; B Ulmasov; W R Folk
Journal:  Plant Mol Biol       Date:  1998-01       Impact factor: 4.076

4.  The nucleotide sequence of a human tRNALeu gene.

Authors:  A McLaren; J P Goddard
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

5.  A human tRNAGlu gene of high transcriptional activity.

Authors:  J P Goddard; M Squire; M Bienz; J D Smith
Journal:  Nucleic Acids Res       Date:  1983-05-11       Impact factor: 16.971

6.  Chromosomal assignment of a glutamic acid transfer RNA (tRNAGlu) gene to 1p36.

Authors:  E Boyd; A Theriault; J P Goddard; M Kalaitsidaki; D H Spathas; J M Connor
Journal:  Hum Genet       Date:  1989-01       Impact factor: 4.132

7.  RNA polymerase III dependence of the human L1 promoter and possible participation of the RNA polymerase II factor YY1 in the RNA polymerase III transcription system.

Authors:  K Kurose; K Hata; M Hattori; Y Sakaki
Journal:  Nucleic Acids Res       Date:  1995-09-25       Impact factor: 16.971

8.  Structure and evolution of a mouse tRNA gene cluster encoding tRNAAsp, tRNAGly and tRNAGlu and an unlinked, solitary gene encoding tRNAAsp.

Authors:  J E Looney; J D Harding
Journal:  Nucleic Acids Res       Date:  1983-12-20       Impact factor: 16.971

9.  Genomic organization of the human asparagine transfer RNA genes: localization to the U1 RNA gene and class I pseudogene repeat units.

Authors:  R A Buckland
Journal:  Am J Hum Genet       Date:  1989-08       Impact factor: 11.025

10.  Construction of an approximately 700-kb transcript map around the familial Mediterranean fever locus on human chromosome 16p13.3.

Authors:  M Centola; X Chen; R Sood; Z Deng; I Aksentijevich; T Blake; D O Ricke; X Chen; G Wood; N Zaks; N Richards; D Krizman; E Mansfield; S Apostolou; J Liu; N Shafran; A Vedula; M Hamon; A Cercek; T Kahan; D Gumucio; D F Callen; R I Richards; R K Moyzis; N A Doggett; F S Collins; P P Liu; N Fischel-Ghodsian; D L Kastner
Journal:  Genome Res       Date:  1998-11       Impact factor: 9.043
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