Literature DB >> 6134271

A human tRNAGlu gene of high transcriptional activity.

J P Goddard, M Squire, M Bienz, J D Smith.   

Abstract

A mixture of low molecular weight RNAs, in which only tRNAs were radiolabelled, was used as a hybridisation probe to select for tRNA-like sequences within a bank of human genomic DNA in lambda Charon 4A. A restriction enzyme digest of one of the selected lambda Charon 4A recombinants contained two fragments (2.4 Kb & 1.8 Kb) which hybridised tRNA and which, when subcloned into pAT153, were transcribed in Xenopus oocyte nuclei. Analysis of the subcloned 2.4 Kb fragment, which was of remarkably high transcriptional activity, revealed the presence of a single gene for tRNAGlu in the middle of the fragment. The sequence immediately preceding the gene has the potential for forming a tRNA-like structure.

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Year:  1983        PMID： 6134271      PMCID： PMC325907          DOI： 10.1093/nar/11.9.2551

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels.

Authors:  A Fradin; H Gruhl; H Feldmann
Journal:  FEBS Lett       Date:  1975-02-01       Impact factor: 4.124

2.  Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.

Authors:  M Silberklang; A M Gillum; U L RajBhandary
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

3.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors:  J G Sutcliffe
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

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Authors:  H Sternbach; F von der Haar; E Schlimme; E Gaertner; F Cramer
Journal:  Eur J Biochem       Date:  1971-09-24

5.  Studies on human tRNA. I. The rapid, large scale isolation and partial fractionation of placenta and liver tRNA.

Authors:  B A Roe
Journal:  Nucleic Acids Res       Date:  1975-01       Impact factor: 16.971

6.  The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.

Authors:  R M Lawn; E F Fritsch; R C Parker; G Blake; T Maniatis
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

7.  Trans-complementable copy-number mutants of plasmid ColE1.

Authors:  A J Twigg; D Sherratt
Journal:  Nature       Date:  1980-01-10       Impact factor: 49.962

8.  Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.

Authors:  A Kressmann; S G Clarkson; V Pirrotta; M L Birnstiel
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

9.  Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

Authors:  S G Clarkson; V Kurer; H O Smith
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

10.  Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.

Authors:  L J Korn; D D Brown
Journal:  Cell       Date:  1978-12       Impact factor: 41.582
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  10 in total

1.  The nucleotide sequence of a human tRNA(Glu) gene.

Authors:  E S Gonos; J P Goddard
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

2.  The role of the 5'-flanking sequence of a human tRNA(Glu) gene in modulation of its transcriptional activity in vitro.

Authors:  E S Gonos; J P Goddard
Journal:  Biochem J       Date:  1990-12-15       Impact factor: 3.857

3.  A human tRNA(iMet) gene produces multiple transcripts.

Authors:  C L Vnencak-Jones; S Z Wahab; Z E Zehner; W M Holmes
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

4.  Chromosomal assignment of a glutamic acid transfer RNA (tRNAGlu) gene to 1p36.

Authors:  E Boyd; A Theriault; J P Goddard; M Kalaitsidaki; D H Spathas; J M Connor
Journal:  Hum Genet       Date:  1989-01       Impact factor: 4.132

5.  Mitotic regulation of a TATA-binding-protein-containing complex.

Authors:  R J White; T M Gottlieb; C S Downes; S P Jackson
Journal:  Mol Cell Biol       Date:  1995-04       Impact factor: 4.272

6.  Genes and pseudogenes in a reiterated rat tRNA gene cluster.

Authors:  A Rosen; S Sarid; V Daniel
Journal:  Nucleic Acids Res       Date:  1984-06-25       Impact factor: 16.971

7.  Transcription factor binding is limited by the 5'-flanking regions of a Drosophila tRNAHis gene and a tRNAHis pseudogene.

Authors:  L Cooley; J Schaack; D J Burke; B Thomas; D Söll
Journal:  Mol Cell Biol       Date:  1984-12       Impact factor: 4.272

8.  Chromosomal assignment of a large tRNA gene cluster (tRNA(Leu), tRNA(Gln), tRNA(Lys), tRNA(Arg), tRNA(Gly)) to 17p13.1.

Authors:  N Morrison; J P Goddard; D H Ledbetter; E Boyd; D Bourn; J M Connor
Journal:  Hum Genet       Date:  1991-06       Impact factor: 4.132

9.  A minor class of 5S rRNA genes in Saccharomyces cerevisiae X2180-1B, one member of which lies adjacent to a Ty transposable element.

Authors:  P W Piper; A Lockheart; N Patel
Journal:  Nucleic Acids Res       Date:  1984-05-25       Impact factor: 16.971

10.  Genomic organization of the human asparagine transfer RNA genes: localization to the U1 RNA gene and class I pseudogene repeat units.

Authors:  R A Buckland
Journal:  Am J Hum Genet       Date:  1989-08       Impact factor: 11.025
  10 in total

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