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Literature DB >> 6308561

Isolation and characterization of genomic mouse DNA clones containing sequences homologous to tRNAs and 5S rRNA.

Abstract

We have cloned and characterized three fragments of Balb/c mouse DNA which hybridize to mouse cell tRNAs. Fractionation of the tRNAs which hybridize to these clones reveals that two of the clones, lambda Mt-4A and lambda Mt-6A hybridize to only one or two tRNAs, while one clone, lambda Mt-4B, hybridizes to at least seven tRNAs. Two of the tRNAs were identified as tRNAProCCG and tRNAGlyGGA, and others have been identified as tRNAs which are selectively encapsidated into virions of murine leukemia virus and avian reticuloendotheliosis virus. The DNA sequences of putative genes for tRNAProCCG and tRNAGlyGGA, plus flanking regions, were determined. A clone of Balb/c mouse DNA which selectively hybridized to 5S rRNA was also isolated and partially characterized.

Entities: Chemical Disease Species

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Year: 1983 PMID： 6308561 PMCID： PMC326087 DOI： 10.1093/nar/11.14.4809

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

31 in total

1. The primer tRNA for Moloney murine leukemia virus DNA synthesis. Nucleotide sequence and aminoacylation of tRNAPro.

Authors: F Harada; G G Peters; J E Dahlberg
Journal: J Biol Chem Date: 1979-11-10 Impact factor: 5.157

2. Plasmids of Escherichia coli as cloning vectors.

Authors: F Bolivar; K Backman
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

3. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors: W D Benton; R W Davis
Journal: Science Date: 1977-04-08 Impact factor: 47.728

4. The isolation of structural genes from libraries of eucaryotic DNA.

Authors: T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal: Cell Date: 1978-10 Impact factor: 41.582

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

6. Sequence of human glycine transfer ribonucleic acid (anticodon CCC). Determination by a newly developed thin-layer readout sequencing technique and comparison with other glycine transfer ribonucleic acids.

Authors: R C Gupta; B A Roe; K Randerath
Journal: Biochemistry Date: 1980-04-15 Impact factor: 3.162

7. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.

Authors: F R Blattner; A E Blechl; K Denniston-Thompson; H E Faber; J E Richards; J L Slightom; P W Tucker; O Smithies
Journal: Science Date: 1978-12-22 Impact factor: 47.728

8. Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.

Authors: F Müller; S G Clarkson
Journal: Cell Date: 1980-02 Impact factor: 41.582

9. Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

Authors: S G Clarkson; V Kurer; H O Smith
Journal: Cell Date: 1978-07 Impact factor: 41.582

10. Low-molecular-weight RNAs of Moloney murine leukemia virus: identification of the primer for RNA-directed DNA synthesis.

Authors: G Peters; F Harada; J E Dahlberg; A Panet; W A Haseltine; D Baltimore
Journal: J Virol Date: 1977-03 Impact factor: 5.103

6 in total

Isolation and characterization of genomic mouse DNA clones containing sequences homologous to tRNAs and 5S rRNA.

1. The primer tRNA for Moloney murine leukemia virus DNA synthesis. Nucleotide sequence and aminoacylation of tRNAPro.

2. Plasmids of Escherichia coli as cloning vectors.

3. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

4. The isolation of structural genes from libraries of eucaryotic DNA.

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

6. Sequence of human glycine transfer ribonucleic acid (anticodon CCC). Determination by a newly developed thin-layer readout sequencing technique and comparison with other glycine transfer ribonucleic acids.

7. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.

8. Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.

9. Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

10. Low-molecular-weight RNAs of Moloney murine leukemia virus: identification of the primer for RNA-directed DNA synthesis.

1. Nucleotide sequence of two proline tRNA (AGG and CGG) genes from chicken.

2. Compilation of tRNA sequences and sequences of tRNA genes.

3. Inhibition of nascent-peptide release at translation termination.

4. Modulation of transcriptional activity and stable complex formation by 5'-flanking regions of mouse tRNAHis genes.

5. Genes and pseudogenes in a reiterated rat tRNA gene cluster.

6. Induction of specific transcription by RNA polymerase III in transformed cells.