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Literature DB >> 105357

Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.

O Schmidt, J I Mao, S Silverman, B Hovemann, D Söll.

Abstract

Cloned tRNA genes from Drosophila and from yeast have been transcribed faithfully in extracts prepared from Xenopus germinal vesicles. The newly formed RNA is composed of precursor tRNAs (of 5S RNA size) and of tRNAs. The plasmid pCIT12 carries genes for Drosophila tRNALys, tRNAArg, and tRNAAsn, Nucleotide analysis of one RNA species transcribed from pCIT12 DNA showed it to be identical to Drosophila tRNALys; it even contained some of the modified nucleotides expected for this tRNA. This RNA species is formed in the germinal vesicle extract via a larger precursor tRNA molecule that does not contain nucleotide modifications. This simple transcription system should aid studies aimed at defining the regulatory DNA regions responsible for eukaryotic gene transcription. In addition, it may provide tRNA precursors that are needed for detailed investigations of eukaryotic tRNA biosynthesis.

Entities: Gene Species

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Year: 1978 PMID： 105357 PMCID： PMC336212 DOI： 10.1073/pnas.75.10.4819

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

20 in total

1. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.

Authors: F Sanger; A R Coulson
Journal: J Mol Biol Date: 1975-05-25 Impact factor: 5.469

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

Authors: D Shortle; D Nathans
Journal: Proc Natl Acad Sci U S A Date: 1978-05 Impact factor: 11.205

10. Escherichia coli RNA polymerase in vitro mimics simian virus 40 in vivo transcription when the template is viral nucleoprotein.

Authors: E B Jakobovits; S Saragosti; M Yaniv; Y Aloni
Journal: Proc Natl Acad Sci U S A Date: 1980-11 Impact factor: 11.205

Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.

1. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

3. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

4. A new method for sequencing DNA.

5. High-fidelity transcription of 5S DNA injected into Xenopus oocytes.

6. A method for the isolation of specific tRNA precursors.

7. Purified DNAs are transcribed after microinjection into Xenopus oocytes.

8. Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.

9. Cloned single repeating units of 5S DNA direct accurate transcription of 5S RNA when injected into Xenopus oocytes.

10. DNA sequencing with chain-terminating inhibitors.

1. The nucleotide sequence of phenylalanine tRNA2 of Drosophila melanogaster: four isoacceptors with one basic sequence.

2. The nucleotide sequence of the initiator tRNA from Drosophila melanogaster.

3. Identification of multiple RNases in Xenopus laevis oocytes and their possible role in tRNA processing.

4. Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract.

5. Genes and pseudogenes in a reiterated rat tRNA gene cluster.

6. Isolation and characterization of cloned rat DNA fragment carrying tRNA genes.

7. Isolation and nucleotide sequence of a plant tRNA gene: petunia asparagine tRNA.

8. Two control regions for eukaryotic tRNA gene transcription.

9. The nucleotide sequence of glutamate tRNA4 of Drosophila melanogaster.

10. Escherichia coli RNA polymerase in vitro mimics simian virus 40 in vivo transcription when the template is viral nucleoprotein.