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Literature DB >> 6256606

Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome.

Abstract

Small multicopy plasmids carrying the Escherichia coli genes ksgA and pdxA were constructed by ligation in vitro of an EcoRI restriction fragment from lambda ksg10 (Andrésson and Davies, 1980a) into the EcoRI sites of the ColE1 plasmids RSF2124 and pVH51. Cleavage maps of the plasmids were determined for 21 different restriction enzymes. The ksgA gene was located in a 750 basepairs (bp) region 1,450 bp clockwise of the EcoRI site in folA: pdxA is in a 2,040 bp region immediately clockwise of ksgA.

Entities: Gene Species

Mesh：

Substances：
DNA Restriction Enzymes

Year: 1980 PMID： 6256606 DOI： 10.1007/bf00268465

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

19 in total

1. Plasmid detection and sizing in single colony lysates.

Authors: W M Barnes
Journal: Science Date: 1977-01-28 Impact factor: 47.728

2. HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene.

Authors: K Postle; W S Reznikoff
Journal: J Bacteriol Date: 1978-12 Impact factor: 3.490

2. The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA.

Authors: S Blanchin-Roland; S Blanquet; J M Schmitter; G Fayat
Journal: Mol Gen Genet Date: 1986-12

Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome.

1. Plasmid detection and sizing in single colony lysates.

2. HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene.

3. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

4. Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12.

5. A simple method for the preparation of large quantities of pure plasmid DNA.

6. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.

7. Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription.

8. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.

9. Transposition of R factor genes to bacteriophage lambda.

10. Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA.

Review 1. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

2. The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA.

3. An unusual genetic link between vitamin B6 biosynthesis and tRNA pseudouridine modification in Escherichia coli K-12.

4. Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12.

Review 5. Linkage map of Escherichia coli K-12, edition 7.

6. Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription.

7. surA, an Escherichia coli gene essential for survival in stationary phase.

8. Identification and characterization of a new gene of Escherichia coli K-12 involved in outer membrane permeability.

9. Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA.