Literature DB >> 318764

Plasmid detection and sizing in single colony lysates.

W M Barnes.   

Abstract

A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 318764     DOI： 10.1126/science.318764

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  70 in total

1.  Filter replicas and permanent collections of recombinant DNA plasmids.

Authors:  J P Gergen; R H Stern; P C Wensink
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

2.  Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.

Authors:  H L Yang; K Heller; M Gellert; G Zubay
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

3.  Short direct repeats flank the T-DNA on a nopaline Ti plasmid.

Authors:  N S Yadav; J Vanderleyden; D R Bennett; W M Barnes; M D Chilton
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

4.  A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.

Authors:  R A Jorgensen; S J Rothstein; W S Reznikoff
Journal:  Mol Gen Genet       Date:  1979

5.  Deletion analysis of the F plasmid oriT locus.

Authors:  Y H Fu; M M Tsai; Y N Luo; R C Deonier
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

6.  Sequence-specific DNA uptake in Haemophilus transformation.

Authors:  K L Sisco; H O Smith
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

7.  Human papillomavirus type 70 genome cloned from overlapping PCR products: complete nucleotide sequence and genomic organization.

Authors:  O Forslund; B G Hansson
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

8.  Cloning of nematode tRNA genes and their expression in the frog oocyte.

Authors:  R Cortese; D Melton; T Tranquilla; J D Smith
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

9.  Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10.

Authors:  R A Jorgensen; W S Reznikoff
Journal:  J Bacteriol       Date:  1979-06       Impact factor: 3.490

10.  Molecular cloning, expression, and characterization of the gene for the surface (HPI)-layer protein of Deinococcus radiodurans in Escherichia coli.

Authors:  J Peters; W Baumeister
Journal:  J Bacteriol       Date:  1986-09       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.