| Literature DB >> 1092355 |
G O Humphreys, G A Willshaw, E S Anderson.
Abstract
Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6, from cleared lysates of plasmid-carrying bacterial strains, After resuspension and density-gradient centrifugation of the precipitated DNA, it is unchanged in length and in transformation efficiency for Escherichia coli K12. Plasmid DNA can be easily prepared in large quantities by including a polyethylene glycol precipitation step in standard plasmid isolation procedures.Entities:
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Year: 1975 PMID： 1092355 DOI： 10.1016/0005-2787(75)90318-4
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002