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Literature DB >> 7005624

Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription.

Abstract

The assay for the ksgA-encoded S-adenosylmethionine--6-N',N'-adenosyl (rRNA) dimethyltransferase has been improved; the gel-filtration molecular weight of partially purified enzyme under two different sets of conditions was found to be 55,000 or 26,000 daltons. We have determined methyltransferase activities in strains where ksgA was brought under the control of the mitomycin C-inducible promoter of the colicin E1 gene. Our studies show that ksgA is transcribed counterclockwise on the Escherichia coli chromosome.

Entities: Chemical Gene Species

Mesh：

Substances：
tRNA Methyltransferases

Year: 1980 PMID： 7005624 DOI： 10.1007/bf00268466

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

19 in total

1. Plasmid detection and sizing in single colony lysates.

Authors: W M Barnes
Journal: Science Date: 1977-01-28 Impact factor: 47.728

2. HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene.

Authors: K Postle; W S Reznikoff
Journal: J Bacteriol Date: 1978-12 Impact factor: 3.490

Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription.

1. Plasmid detection and sizing in single colony lysates.

2. HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene.

Review 3. Plasmid-determined resistance to antimicrobial agents.

4. Characterization of in vitro transcription initiation and termination sites in Col E1 DNA.

5. Studies on ribosome structure and interactions near the m62Am62A sequence.

6. Mechanism of kasugamycin resistance in Escherichia coli.

7. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.

8. Malate dehydrogenases. I. A survey of molecular size measured by gel filtration.

9. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.

10. Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA.

Review 1. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

2. Site and substrate specificity of the ermC 23S rRNA methyltransferase.

3. Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome.

Review 4. Linkage map of Escherichia coli K-12, edition 7.

5. MRM2 encodes a novel yeast mitochondrial 21S rRNA methyltransferase.

6. surA, an Escherichia coli gene essential for survival in stationary phase.

7. ksgA mutations confer resistance to kasugamycin in Neisseria gonorrhoeae.

8. Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance.

9. Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA.