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Literature DB >> 6450312

Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA.

Abstract

Lambda phages carrying the Escherichia coli genes ksgA and pdxA were isolated from secondary site lysogens in araB. 1) The phage genomes were characterized by genetic complementation tests, restriction endonuclease digestion and electron microscopy. 2) A 6.3 kilobasepair (kb) EcoRI restriction fragment carrying both ksgA and pdxA was cloned in a lambda vector; this fragment has proven useful in further characterization of the ksgA gene (Andrésson and Davies, 1980a, b). The ksgA and pdxA genes are about 14 and 12-13 kb, respectively, counterclockwise of the arabinose operon and 1.5 and 2.5-3.5 kb clockwise of folA.

Entities: Gene Species

Mesh：

Year: 1980 PMID： 6450312 DOI： 10.1007/bf00268464

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

29 in total

1. Nucleotide sequence of the 5' end of araBAD operon messenger RNA in Escherichia coli B/r.

Authors: N Lee; J Carbon
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

2. Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes.

Authors: R J Legerski; J L Hodnett; H B Gray
Journal: Nucleic Acids Res Date: 1978-05 Impact factor: 16.971

3. HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene.

Authors: K Postle; W S Reznikoff
Journal: J Bacteriol Date: 1978-12 Impact factor: 3.490

4. Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli.

Authors: S H Thorbjarnardóttir; R A Magnúsdóttir; G Eggertsson
Journal: Mol Gen Genet Date: 1978-04-25

5. Mechanism of kasugamycin resistance in Escherichia coli.

Authors: T L Helser; J E Davies; J E Dahlberg
Journal: Nat New Biol Date: 1972-01-05

6. Isolation of specialized transducing bacteriophage lambda carrying genes of the L-arabinose operon of Escherichia coli B/r.

Authors: J Boulter; N Lee
Journal: J Bacteriol Date: 1975-09 Impact factor: 3.490

7. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.

Authors: J A Steitz; K Jakes
Journal: Proc Natl Acad Sci U S A Date: 1975-12 Impact factor: 11.205

8. Identification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome.

Authors: S R Jaskunas; R R Burgess; M Nomura
Journal: Proc Natl Acad Sci U S A Date: 1975-12 Impact factor: 11.205

Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA.

1. Nucleotide sequence of the 5' end of araBAD operon messenger RNA in Escherichia coli B/r.

2. Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes.

3. HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene.

4. Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli.

5. Mechanism of kasugamycin resistance in Escherichia coli.

6. Isolation of specialized transducing bacteriophage lambda carrying genes of the L-arabinose operon of Escherichia coli B/r.

7. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.

8. Identification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome.

9. Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.

10. Potassium transport loci in Escherichia coli K-12.

Review 1. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

2. Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome.

Review 3. Linkage map of Escherichia coli K-12, edition 7.

4. Some properties of the ribosomal RNA methyltransferase encoded by ksgA and the polarity of ksgA transcription.

5. Salmonella enterica serovar typhimurium surA mutants are attenuated and effective live oral vaccines.

6. surA, an Escherichia coli gene essential for survival in stationary phase.