Literature DB >> 62361

Human somatotropin: biological characterization of the recombinant molecule.

C H Li, T Hayashida, B A Doneen, A J Rao.   

Abstract

The recombinant hormone obtained by non-covalent interaction of the NH2-terminal 134 amino acid fragment with the COOH-terminal 51 amino acid fragment of the reduced-carbamidomethylated human somatotropin molecule is found to exhibit nearly full biological activity of the native hormone, as evidenced by the stimulation of hepatic ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) in vivo and protein synthesis in mouse mammary gland in vitro. Radioimmunoassay data indicate that the recombinant behaves immunochemically in a manner almost identical to that of the native hormone.

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Year:  1976        PMID： 62361      PMCID： PMC431135          DOI： 10.1073/pnas.73.10.3463

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  Isolation and biological characterization of fragments of human growth hormone produced by digestion with plasmin.

Authors:  C R Reagan; J B Mills; J L Kostyo; A E Wilhelmi
Journal:  Endocrinology       Date:  1975-03       Impact factor: 4.736

2.  THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors:  F C GREENWOOD; W M HUNTER; J S GLOVER
Journal:  Biochem J       Date:  1963-10       Impact factor: 3.857

3.  Human pituitary growth hormone. VI. Modified procedure of isolation and NH2-terminal amino acid sequence.

Authors:  C H LI; W K LIU; J S DIXON
Journal:  Arch Biochem Biophys       Date:  1962-09       Impact factor: 4.013

4.  Isohormones of human growth hormone. II. Plasmin-catalyzed transformation and increase in prolactin biological activity.

Authors:  R A Yadley; A Chrambach
Journal:  Endocrinology       Date:  1973-10       Impact factor: 4.736

5.  Human pituitary growth hormone: immunochemical investigations of biologically active fragments.

Authors:  W C Clarke; T Hayashida; C H Li
Journal:  Arch Biochem Biophys       Date:  1974-10       Impact factor: 4.013

6.  On the purification of L-ornithine decarboxylase from rat prostate and effects of thiol compounds on the enzyme.

Authors:  J Jänne; H G Williams-Ashman
Journal:  J Biol Chem       Date:  1971-03-25       Impact factor: 5.157

7.  Human pituitary growth hormone. 44. Effects of plasmin-modified hormone and its fragments on ornithine decarboxylase activity and lipolysis.

Authors:  A J Rao; J Ramachandran; C H Li
Journal:  Proc Soc Exp Biol Med       Date:  1976-02

8.  Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.

Authors:  C H Li; T A Bewley
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

9.  Human pituitary growth hormone: isolation and properties of two biologically active fragments from plasmin digests.

Authors:  C H Li; L Gráf
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205

10.  Metabolic effects of plasmin digests of human growth hormone in the rat and man.

Authors:  J B Mills; C R Reagan; D Rudman; J L Kostyo; P Zachariah; A E Wilhelmi
Journal:  J Clin Invest       Date:  1973-11       Impact factor: 14.808
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  5 in total

1.  Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.

Authors:  Y Birk; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

2.  Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.

Authors:  C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

3.  Semisynthesis of human somatotropin analogs.

Authors:  C H Li; J Blake
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

Review 4.  Human growth hormone: 1974-1981.

Authors:  C H Li
Journal:  Mol Cell Biochem       Date:  1982-07-07       Impact factor: 3.396

5.  Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.

Authors:  C H Li; T A Bewley; J Blake; T Hayashida
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205
  5 in total

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