Literature DB >> 273900

Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.

C H Li.   

Abstract

Complementation of the plasmin fragments of reduced-carbamoylmethylated (Cam) human somatotropin (hGH) with those of reduced-carbamoylmethylated human chorionic somatomammotropin (hCS) have been investigated. It was found that the recombinant obtained by noncovalent interaction of [Cys(Cam)53]hGH-(1-134) with [Cys(Cam)-165,182,189]hCS-(141-191) exhibits 50% growth-promoting activity and nearly full immunoreactivity. Complementation of [Cys(Cam)53]hCS-(1-133) with the COOH-terminal fragment of hGH generated lower growth-promoting and immunological activities.

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Year:  1978        PMID： 273900      PMCID： PMC392406          DOI： 10.1073/pnas.75.4.1700

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  Bioassay of hypophyseal growth hormone; the tibia test.

Authors:  F S GREENSPAN; C H LI
Journal:  Endocrinology       Date:  1949-11       Impact factor: 4.736

2.  Human pituitary growth hormone: immunochemical investigations of biologically active fragments.

Authors:  W C Clarke; T Hayashida; C H Li
Journal:  Arch Biochem Biophys       Date:  1974-10       Impact factor: 4.013

3.  Amino acid sequence of human chorionic somatomammotropin.

Authors:  C H Li; J S Dixon; D Chung
Journal:  Arch Biochem Biophys       Date:  1973-03       Impact factor: 4.013

4.  Plasma growth hormone concentration in the rat determined by radioimmunoassay: influence of sex, pregnancy, lactation, anesthesia, hypophysectomy and extrasellar pituitary transplants.

Authors:  D S Schalch; S Reichlin
Journal:  Endocrinology       Date:  1966-08       Impact factor: 4.736

5.  Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.

Authors:  C H Li; T A Bewley
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

6.  Human pituitary growth hormone: isolation and properties of two biologically active fragments from plasmin digests.

Authors:  C H Li; L Gráf
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205

7.  Human somatotropin 53. Synthesis and biological activity of the amino terminal fifty-four residue fragment.

Authors:  R L Noble; D Yamashiro; C H Li
Journal:  Int J Pept Protein Res       Date:  1977-11

8.  Human somatotropin: biological characterization of the recombinant molecule.

Authors:  C H Li; T Hayashida; B A Doneen; A J Rao
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

9.  Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.

Authors:  C H Li; T A Bewley; J Blake; T Hayashida
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205
  9 in total
  2 in total

1.  Semisynthesis of human somatotropin analogs.

Authors:  C H Li; J Blake
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

2.  Immunoreactivity and receptor binding of mixed recombinants of human growth hormone and chorionic somatomammotropin.

Authors:  S Burstein; M M Grumbach; S L Kaplan; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205
  2 in total

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