Literature DB >> 58417

Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.

C H Li, T A Bewley.   

Abstract

The NH2-terminal 134 amino-acid fragment of the reduced-carbamidomethylated human somatropin molecule is found to react noncovalently with the COOH-terminal 51 amino-acid fragment in solutions of pH 8.4 at 2 degrees to restore full biological activity as evidenced by the rat tibia and pigeon crop-sac assays. In addition, circular dichroism spectra of the recombinant show the conformation to be completely repaired in comparison with that of the native hormone.

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Year:  1976        PMID： 58417      PMCID： PMC430319          DOI： 10.1073/pnas.73.5.1476

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  Isolation and biological characterization of fragments of human growth hormone produced by digestion with plasmin.

Authors:  C R Reagan; J B Mills; J L Kostyo; A E Wilhelmi
Journal:  Endocrinology       Date:  1975-03       Impact factor: 4.736

Review 2.  Protein complementation.

Authors:  I Zabin; M R Villarejo
Journal:  Annu Rev Biochem       Date:  1975       Impact factor: 23.643

3.  The synthesis and biological activity of (165, 182, 189-S-carbamidomethylcysteine)-human growth hormone-(140-191).

Authors:  J Blake; C H Li
Journal:  Int J Pept Protein Res       Date:  1975

4.  MOLECULAR WEIGHT OF SHEEP PITUITARY INTERSTITIAL CELL-STIMULATING HORMONE.

Authors:  C H LI; B STARMAN
Journal:  Nature       Date:  1964-04-18       Impact factor: 49.962

5.  Human pituitary growth hormone. VI. Modified procedure of isolation and NH2-terminal amino acid sequence.

Authors:  C H LI; W K LIU; J S DIXON
Journal:  Arch Biochem Biophys       Date:  1962-09       Impact factor: 4.013

6.  Bioassay of hypophyseal growth hormone; the tibia test.

Authors:  F S GREENSPAN; C H LI
Journal:  Endocrinology       Date:  1949-11       Impact factor: 4.736

Review 7.  Experimental and theoretical aspects of protein folding.

Authors:  C B Anfinsen; H A Scheraga
Journal:  Adv Protein Chem       Date:  1975

8.  Identity between the growth hormone degrading activity of the pituitary gland and plasmin.

Authors:  S Ellis; J M Nuenke; R E Grindeland
Journal:  Endocrinology       Date:  1968-11       Impact factor: 4.736

9.  Isohormones of human growth hormone. II. Plasmin-catalyzed transformation and increase in prolactin biological activity.

Authors:  R A Yadley; A Chrambach
Journal:  Endocrinology       Date:  1973-10       Impact factor: 4.736

10.  Metabolic effects of plasmin digests of human growth hormone in the rat and man.

Authors:  J B Mills; C R Reagan; D Rudman; J L Kostyo; P Zachariah; A E Wilhelmi
Journal:  J Clin Invest       Date:  1973-11       Impact factor: 14.808
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  15 in total

1.  In vivo assembly of aspartate transcarbamoylase from fragmented and circularly permuted catalytic polypeptide chains.

Authors:  X Ni; H K Schachman
Journal:  Protein Sci       Date:  2001-03       Impact factor: 6.725

2.  Functional assembly of a randomly cleaved protein.

Authors:  K Shiba; P Schimmel
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

3.  Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.

Authors:  Y Birk; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

4.  Preparation of a disulfide-linked dimer of human placental lactogen fragment 1--134 with immunologic and biologic activity.

Authors:  J Russell; L M Sherwood; K Kowalski; A B Schneider
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

5.  Rate of concentration and digestion of radioactive growth hormone preparations injected in rats, as measured by the amount and nature of radioactivity in the tissues.

Authors:  L A Retegui-Sardou; L O Scaramal; J M Dellacha; A C Paladini
Journal:  Mol Cell Biochem       Date:  1977-07-05       Impact factor: 3.396

6.  Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.

Authors:  C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

7.  Semisynthesis of human somatotropin analogs.

Authors:  C H Li; J Blake
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

8.  Assembly of functional Escherichia coli RNA polymerase containing beta subunit fragments.

Authors:  K Severinov; A Mustaev; E Severinova; I Bass; M Kashlev; R Landick; V Nikiforov; A Goldfarb; S A Darst
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-09       Impact factor: 11.205

9.  Activity of human growth hormone and related polypeptides on the adipose conversion of 3T3 cells.

Authors:  M Morikawa; H Green; U J Lewis
Journal:  Mol Cell Biol       Date:  1984-02       Impact factor: 4.272

10.  A single domain of human prostatic acid phosphatase shows antibody-mediated restoration of catalytic activity.

Authors:  B K Choe; M K Dong; D Walz; S Gleason; N R Rose
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205
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