Literature DB >> 149984

Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.

Y Birk, C H Li.   

Abstract

The action of fibrinolysin (plasmin; EC 3.4.21.7) on ovine prolactin has been investigated. It was found that the enzyme selectively cleaves the bond between Met-53 and Ala-54. The two fragments, PRL-(1-53) and PRL-(54-199), have been purified and characterized. A recombinant molecule has been obtained by noncovalent interaction of PRL-(1-53) and PRL-(54-199). The recombined protein behaves nearly identically to the parent hormone in circular dichroism spectra and exclusion chromatography. The recombinant possesses full immunoreactivity, as revealed by gel double-diffusion and complement fixation. However, the recombined protein exhibits low prolactin activity in the pigeon crop-sac test.

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Year:  1978        PMID： 149984      PMCID： PMC392510          DOI： 10.1073/pnas.75.5.2155

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition.

Authors:  E WASSERMAN; L LEVINE
Journal:  J Immunol       Date:  1961-09       Impact factor: 5.422

3.  Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion.

Authors:  O OUCHTERLONY
Journal:  Acta Pathol Microbiol Scand       Date:  1953

4.  Studies on pituitary lactogenic hormone. The primary structure of the porcine hormone.

Authors:  C H Li
Journal:  Int J Pept Protein Res       Date:  1976

5.  Circular dichroism studies on human pituitary growth hormone and ovine pituitary lactogenic hormone.

Authors:  T A Bewley; C H Li
Journal:  Biochemistry       Date:  1972-02-29       Impact factor: 3.162

6.  Comparative studies of the single tryptophan residue in human chorionic somatomammotropin and human pituitary growth hormone.

Authors:  T A Bewley; H Kawauchi; C H Li
Journal:  Biochemistry       Date:  1972-10-24       Impact factor: 3.162

7.  Studies on pituitary lactogenic hormone. XXX. The primary structure of the sheep hormone.

Authors:  C H Li; J S Dixon; T B Lo; K D Schmidt; Y A Pankov
Journal:  Arch Biochem Biophys       Date:  1970-12       Impact factor: 4.013

8.  Complete amino acid analysis of proteins from a single hydrolysate.

Authors:  R J Simpson; M R Neuberger; T Y Liu
Journal:  J Biol Chem       Date:  1976-04-10       Impact factor: 5.157

9.  Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.

Authors:  C H Li; T A Bewley
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

10.  Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.

Authors:  C H Li; T A Bewley; J Blake; T Hayashida
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205
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  3 in total

1.  A single domain of human prostatic acid phosphatase shows antibody-mediated restoration of catalytic activity.

Authors:  B K Choe; M K Dong; D Walz; S Gleason; N R Rose
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

2.  Radioimmunoreactivity and receptor-binding activity of the recombined molecule obtained by complementation of two fibrinolysin fragments of ovine prolactin.

Authors:  T M Wong; C H Cheng; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

3.  Ovine prolactin: equilibrium characteristics of the recombinant molecule formed by noncovalent interaction of two fibrinolysin fragments by fluorescence polarization.

Authors:  M D Jibson; C H Li; C B Glaser
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205
  3 in total

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