Literature DB >> 6196967

Fragile X expression is decreased by 5-azacytidine and S-adenosylhomocysteine.

J C Mixon, V G Dev.   

Abstract

A lymphoblastoid cell line derived from a patient with fragile X chromosome exhibited fragility only when 5-fluoro-2'-deoxyuridine (FUdR) was added to the culture medium. Addition of methionine with FUdR greatly increased the frequency of fragile X. Addition of 5-azacytidine (an inhibitor of methylation at the level of DNA) or S-adenosylhomocysteine (an inhibitor of the synthesis of the methyl group donor S-adenosylmethionine) reversed the effect of methionine as well as that of FUdR. It is proposed that DNA methylation is in some way involved in the mechanism of fragile X expression.

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Year:  1983        PMID： 6196967      PMCID： PMC1685956     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  9 in total

1.  Heritable fragile sites on human chromosomes I. Factors affecting expression in lymphocyte culture.

Authors:  G R Sutherland
Journal:  Am J Hum Genet       Date:  1979-03       Impact factor: 11.025

2.  Inhibition of methylation by adenosine in adenosine deaminase-inhibited, phytohemagglutinin-stimulated human lymphocytes.

Authors:  J M Johnston; N M Kredich
Journal:  J Immunol       Date:  1979-07       Impact factor: 5.422

3.  Expression of the marker (X) (q28) in lymphoblastoid cell lines.

Authors:  P A Jacobs; P A Hunt; M Mayer; J C Wang; G R Boss; R W Erbe
Journal:  Am J Hum Genet       Date:  1982-07       Impact factor: 11.025

Review 4.  DNA methylation--a regulatory signal in eukaryotic gene expression.

Authors:  W Doerfler
Journal:  J Gen Virol       Date:  1981-11       Impact factor: 3.891

5.  5-Fluoro-2'-deoxyuridine induction of the fragile site on Xq28 associated with X linked mental retardation.

Authors:  N Tommerup; H Poulsen; K Brøndum-Nielsen
Journal:  J Med Genet       Date:  1981-10       Impact factor: 6.318

6.  Cellular differentiation, cytidine analogs and DNA methylation.

Authors:  P A Jones; S M Taylor
Journal:  Cell       Date:  1980-05       Impact factor: 41.582

7.  Fragile sites in human chromosomes I. The effect of methionine on the Xq fragile site.

Authors:  P N Howard-Peebles; J C Pryor
Journal:  Clin Genet       Date:  1981-04       Impact factor: 4.438

8.  The combined effects of FUdR addition and methionine depletion on the X-chromosome fragile site.

Authors:  T W Glover; P N Howard-Peebles
Journal:  Am J Hum Genet       Date:  1983-01       Impact factor: 11.025

9.  FUdR induction of the X chromosome fragile site: evidence for the mechanism of folic acid and thymidine inhibition.

Authors:  T W Glover
Journal:  Am J Hum Genet       Date:  1981-03       Impact factor: 11.025
  9 in total
  4 in total

1.  The effect of methionine and 5-azacytidine on fragile X expression.

Authors:  M A Abruzzo; M Mayer; P A Jacobs
Journal:  Am J Hum Genet       Date:  1985-01       Impact factor: 11.025

2.  DNA demethylation induced by 5-azacytidine does not affect fragile X expression.

Authors:  T W Glover; J Coyle-Morris; L Pearce-Birge; C Berger; R M Gemmill
Journal:  Am J Hum Genet       Date:  1986-03       Impact factor: 11.025

3.  Fragile X syndrome: clinical, cytogenetic, biochemical and molecular features.

Authors:  J C Mixon; V G Dev
Journal:  Indian J Pediatr       Date:  1986 Jul-Aug       Impact factor: 1.967

4.  CGG repeats associated with DNA instability and chromosome fragility form structures that block DNA synthesis in vitro.

Authors:  K Usdin; K J Woodford
Journal:  Nucleic Acids Res       Date:  1995-10-25       Impact factor: 16.971
  4 in total

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