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Literature DB >> 2579550

The effect of methionine and 5-azacytidine on fragile X expression.

Abstract

The cellular mechanism for the expression of the fragile site at Xq28 is unknown. We tested the effect of 5-azacytidine and methionine on fragile X expression in lymphocytes and lymphoblastoid cells in an attempt to determine if DNA methylation was involved. We were unable to demonstrate a consistent dosage effect of methionine on fragile X expression. While 5-azacytidine was found to inhibit the fragile X in both males and females, it did so only at relatively high concentrations. We conclude that the role, if any, of DNA methylation in fragile X expression is likely to be secondary, the primary effect being due to thymidylate depletion.

Entities: Chemical

Mesh：

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Year: 1985 PMID： 2579550 PMCID： PMC1684555

Source DB: PubMed Journal: Am J Hum Genet ISSN： 0002-9297 Impact factor: 11.025

15 in total

1. Fragile sites on human chromosomes: demonstration of their dependence on the type of tissue culture medium.

Authors: G R Sutherland
Journal: Science Date: 1977-07-15 Impact factor: 47.728

2. Fragile X chromosome: consistent demonstration of fragile site in fibroblast cultures.

Authors: A P Gardner; R T Howell; A McDermott
Journal: Lancet Date: 1982-01-09 Impact factor: 79.321

3. Folate metabolism in humans.

Authors: R W Erbe; J C Wang
Journal: Am J Med Genet Date: 1984-01

4. Expression of the marker (X) (q28) in lymphoblastoid cell lines.

Authors: P A Jacobs; P A Hunt; M Mayer; J C Wang; G R Boss; R W Erbe
Journal: Am J Hum Genet Date: 1982-07 Impact factor: 11.025

Review 5. The fragile X chromosome.

Authors: G R Sutherland
Journal: Int Rev Cytol Date: 1983

3. 2017 Presidential Address: Checking, Balancing, and Celebrating Diversity: Celebrating Some of the Women Who Paved the Way.

Authors: Nancy J Cox
Journal: Am J Hum Genet Date: 2018-03-01 Impact factor: 11.025

3 in total

The effect of methionine and 5-azacytidine on fragile X expression.

1. Fragile sites on human chromosomes: demonstration of their dependence on the type of tissue culture medium.

2. Fragile X chromosome: consistent demonstration of fragile site in fibroblast cultures.

3. Folate metabolism in humans.

4. Expression of the marker (X) (q28) in lymphoblastoid cell lines.

Review 5. The fragile X chromosome.

6. [Metabolism of monocarbons and the fragile X syndrome].

7. On the nature of folic-acid-sensitive fragile sites in human chromosomes: an hypothesis.

8. 5-Azacytidine-induced reactivation of a herpes simplex thymidine kinase gene.

9. Fragile sites in human chromosomes I. The effect of methionine on the Xq fragile site.

10. The combined effects of FUdR addition and methionine depletion on the X-chromosome fragile site.

1. DNA demethylation induced by 5-azacytidine does not affect fragile X expression.

2. Fragile X syndrome: clinical, cytogenetic, biochemical and molecular features.

3. 2017 Presidential Address: Checking, Balancing, and Celebrating Diversity: Celebrating Some of the Women Who Paved the Way.