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Literature DB >> 61588

A general method for cloning eukaryotic structural gene sequences.

R Higuchi, G V Paddock, R Wall, W Salser.

Abstract

Complementary DNA, transcribed in vitro from purified rabbit globin messenger RNA and made double-stranded, has been inserted into Escherichia coli plasmids pSC101 and pMB9 by the poly(dT)/poly(dA) "tailing" and annealing technique. E. coli transformants given by this DNA preparation have been shown to contain globin sequences by the hybridization of globin RNA to DNA from clones grown and lysed in situ on nitrocellulose filters. An estimate of the amount of inserted globin sequences has been provided by fingerprint analysis of globin mRNA sequences hybridized to the purified plasmid chimeras. Inserted sequences so far subjected to detailed analysis have been ascribed to the rabbit beta globin chain. The susceptibility of inserted beta globin, sequences to the restriction endonuclease EcoRI confirms the existence of a site already found through previous nucleotide sequence analysis.

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Year: 1976 PMID： 61588 PMCID： PMC430960 DOI： 10.1073/pnas.73.9.3146

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

24 in total

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Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

Review 5. DNA sequencing techniques.

Authors: W A Salser
Journal: Annu Rev Biochem Date: 1974 Impact factor: 23.643

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Journal: Proc Natl Acad Sci U S A Date: 1974-09 Impact factor: 11.205

10. Replication and transcription of eukaryotic DNA in Escherichia coli.

Authors: J F Morrow; S N Cohen; A C Chang; H W Boyer; H M Goodman; R B Helling
Journal: Proc Natl Acad Sci U S A Date: 1974-05 Impact factor: 11.205

32 in total

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Journal: Proc Natl Acad Sci U S A Date: 1978-12 Impact factor: 11.205

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Journal: Nucleic Acids Res Date: 1978-09 Impact factor: 16.971

5. The rat serum albumin gene: analysis of cloned sequences.

Authors: T D Sargent; J R Wu; J M Sala-Trepat; R B Wallace; A A Reyes; J Bonner
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6. Quantitative assessment of actin transcript number in eggs, embryos, and tube feet of the sea star Pisaster ochraceus.

Authors: I Kovesdi; M J Smith
Journal: Mol Cell Biol Date: 1985-11 Impact factor: 4.272

7. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.

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