Literature DB >> 1101952

A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.

R R Burgess, J J Jendrisak.   

Abstract

An improved method is described for the purification of the DNA-dependent RNA polymerase [ribonucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6] from Escherichia coli. The method involves lysozyme-sodium deoxycholate lysis, low-speed centrifugation, precipitation with Polymin P, elution from the Polymin P precipitate, ammonium sulfate precipitation, and chromatography on DNA-cellulose and Bio-Gel A 5m. RNA polymerase is purified to electrophoretic homogeneity in 2 days with a recovery of 45%, resulting in a yield of 250 mg of holoenzyme from 500 g of cells.

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Year:  1975        PMID： 1101952     DOI： 10.1021/bi00692a011

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  369 in total

1.  The interface of sigma with core RNA polymerase is extensive, conserved, and functionally specialized.

Authors:  M M Sharp; C L Chan; C Z Lu; M T Marr; S Nechaev; E W Merritt; K Severinov; J W Roberts; C A Gross
Journal:  Genes Dev       Date:  1999-11-15       Impact factor: 11.361

2.  Escherichia coli RNA polymerase core and holoenzyme structures.

Authors:  R D Finn; E V Orlova; B Gowen; M Buck; M van Heel
Journal:  EMBO J       Date:  2000-12-15       Impact factor: 11.598

3.  A long T. A tract in the upp initially transcribed region is required for regulation of upp expression by UTP-dependent reiterative transcription in Escherichia coli.

Authors:  Y Cheng; S M Dylla; C L Turnbough
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

4.  Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells.

Authors:  J Zhu; S C Winans
Journal:  Proc Natl Acad Sci U S A       Date:  1999-04-27       Impact factor: 11.205

5.  Coactivation of the RpoS-dependent proP P2 promoter by fis and cyclic AMP receptor protein.

Authors:  S M McLeod; J Xu; R C Johnson
Journal:  J Bacteriol       Date:  2000-08       Impact factor: 3.490

6.  Dissecting the functional program of Escherichia coli promoters: the combined mode of action of Lac repressor and AraC activator.

Authors:  R Lutz; T Lozinski; T Ellinger; H Bujard
Journal:  Nucleic Acids Res       Date:  2001-09-15       Impact factor: 16.971

7.  Interaction of RNA polymerase with forked DNA: evidence for two kinetically significant intermediates on the pathway to the final complex.

Authors:  Laura Tsujikawa; Oleg V Tsodikov; Pieter L deHaseth
Journal:  Proc Natl Acad Sci U S A       Date:  2002-03-12       Impact factor: 11.205

8.  Formation of intermediate transcription initiation complexes at pfliD and pflgM by sigma(28) RNA polymerase.

Authors:  J R Givens; C L McGovern; A J Dombroski
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

9.  Topography of lacUV5 initiation complexes.

Authors:  V Studitsky; K Brodolin; Y Liu; A Mirzabekov
Journal:  Nucleic Acids Res       Date:  2001-02-01       Impact factor: 16.971

10.  Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior.

Authors:  Karen Adelman; Arthur La Porta; Thomas J Santangelo; John T Lis; Jeffrey W Roberts; Michelle D Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-07       Impact factor: 11.205
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