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Literature DB >> 4139714

Nucleotide sequence analysis of RNA synthesized from rabbit globin complementary DNA.

R Poon, G V Paddock, H Heindell, P Whitcome, W Salser, D Kacian, A Bank, R Gambino, F Ramirez.

Abstract

Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of (32)P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T(1) and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the alpha or beta globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants.

Entities: Chemical Gene Species

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Year: 1974 PMID： 4139714 PMCID： PMC433802 DOI： 10.1073/pnas.71.9.3502

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

1. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).

Authors: J Ross; H Aviv; E Scolnick; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1972-01 Impact factor: 11.205

2. Synthesis of DNA complements of natural RNAs: a general approach.

Authors: S Spiegelman; K F Watson; D L Kacian
Journal: Proc Natl Acad Sci U S A Date: 1971-11 Impact factor: 11.205

3. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

Authors: I M Verma; G F Temple; H Fan; D Baltimore
Journal: Nat New Biol Date: 1972-02-09

4. RNA-dependent DNA polymerase in virions of RNA tumour viruses.

Authors: D Baltimore
Journal: Nature Date: 1970-06-27 Impact factor: 49.962

5. RNA-dependent DNA polymerase in virions of Rous sarcoma virus.

Authors: H M Temin; S Mizutani
Journal: Nature Date: 1970-06-27 Impact factor: 49.962

6. Separation and characterization of the subunits of ribonucleic acid polymerase.

Authors: R R Burgess
Journal: J Biol Chem Date: 1969-11-25 Impact factor: 5.157

7. Topography of nucleic acid helices in solutions. IV. Effect of polyamines on RNase-catalyzed hydrolysis of polyadenylic acid.

Authors: E J Gabbay; R R Shimshak
Journal: Biopolymers Date: 1968 Impact factor: 2.505

8. A two-dimensional fractionation procedure for radioactive nucleotides.

Authors: F Sanger; G G Brownlee; B G Barrell
Journal: J Mol Biol Date: 1965-09 Impact factor: 5.469

9. Isolation of an RNA with the properties of haemoglobin messenger.

Authors: F Labrie
Journal: Nature Date: 1969-03-29 Impact factor: 49.962

10. Properties of isoaccepting species of lysine tRNA from rabbit reticulocytes in codon recognition and in haemoglobin biosynthesis in vitro.

Authors: E Rudloff; K Hilse
Journal: Eur J Biochem Date: 1971-12

9 in total

Nucleotide sequence analysis of RNA synthesized from rabbit globin complementary DNA.

1. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).

2. Synthesis of DNA complements of natural RNAs: a general approach.

3. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

4. RNA-dependent DNA polymerase in virions of RNA tumour viruses.

5. RNA-dependent DNA polymerase in virions of Rous sarcoma virus.

6. Separation and characterization of the subunits of ribonucleic acid polymerase.

7. Topography of nucleic acid helices in solutions. IV. Effect of polyamines on RNase-catalyzed hydrolysis of polyadenylic acid.

8. A two-dimensional fractionation procedure for radioactive nucleotides.

9. Isolation of an RNA with the properties of haemoglobin messenger.

10. Properties of isoaccepting species of lysine tRNA from rabbit reticulocytes in codon recognition and in haemoglobin biosynthesis in vitro.

1. Recombinant DNA clones constructed from immunoglobulin kappa light chain messenger RNA.

2. Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison.

3. Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene.

4. Deoxysubstitution in RNA by RNA polymerase in vitro: a new approach to nucleotide sequence determinations.

5. Strand-selective transcription of globin genes in rabbit erythroid cells and chromatin.

6. Nucleotide sequence from the coding region of rabbit beta-globin messenger RNA.

7. The syntheiss of high yields of full-length reverse transcripts of globin mRNA.

8. The nucleotide sequences of the untranslated 5' regions of human alpha- and beta-globin mRNAs.

9. A general method for cloning eukaryotic structural gene sequences.