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Literature DB >> 4594039

Construction of biologically functional bacterial plasmids in vitro.

S N Cohen, A C Chang, H W Boyer, R B Helling.

Abstract

The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genetic properties and nucleotide base sequences from both of the parent DNA molecules. Functional plasmids can be obtained by reassociation of endonuclease-generated fragments of larger replicons, as well as by joining of plasmid DNA molecules of entirely different origins.

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Year: 1973 PMID： 4594039 PMCID： PMC427208 DOI： 10.1073/pnas.70.11.3240

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

11 in total

1. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

Authors: P A Sharp; M T Hsu; E Otsubo; N Davidson
Journal: J Mol Biol Date: 1972-11-14 Impact factor: 5.469

2. Recognition sequence of a restriction enzyme.

Authors: C H Bigger; K Murray; N E Murray
Journal: Nat New Biol Date: 1973-07-04

3. DNA nucleotide sequence restricted by the RI endonuclease.

Authors: J Hedgpeth; H M Goodman; H W Boyer
Journal: Proc Natl Acad Sci U S A Date: 1972-11 Impact factor: 11.205

4. Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends.

Authors: J E Mertz; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1972-11 Impact factor: 11.205

5. DNA substrate site for the EcoRII restriction endonuclease and modification methylase.

Authors: H W Boyer; L T Chow; A Dugaiczyk; J Hedgpeth; H M Goodman
Journal: Nat New Biol Date: 1973-07-11

6. The problems of drug-resistant pathogenic bacteria. R factors: biochemical mechanisms of resistance to aminoglycoside antibiotics.

Authors: J Davies; M Brzezinska; R Benveniste
Journal: Ann N Y Acad Sci Date: 1971-06-11 Impact factor: 5.691

7. Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli.

Authors: S N Cohen; C A Miller
Journal: J Mol Biol Date: 1970-06-28 Impact factor: 5.469

8. Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium.

Authors: E S Anderson; M J Lewis
Journal: Nature Date: 1965-11-27 Impact factor: 49.962

9. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors: S N Cohen; A C Chang; L Hsu
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

10. Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants.

Authors: S N Cohen; A C Chang
Journal: Proc Natl Acad Sci U S A Date: 1973-05 Impact factor: 11.205

399 in total

Review 1. Molecular techniques in biomedical sciences: a new era in diagnosis of infectious diseases.

Authors: S Chandwani; A Kaul
Journal: Indian J Pediatr Date: 1995 Jan-Feb Impact factor: 1.967

2. Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

Authors: R A Skurray; H Nagaishi; A J Clark
Journal: Proc Natl Acad Sci U S A Date: 1976-01 Impact factor: 11.205

3. Class of small multicopy plasmids originating from the mutant antibiotic resistance factor R1 drd-19B2.

Authors: W Goebel; R Bonewald
Journal: J Bacteriol Date: 1975-08 Impact factor: 3.490

4. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.

Authors: D J Kopecko; S N Cohen
Journal: Proc Natl Acad Sci U S A Date: 1975-04 Impact factor: 11.205

5. Cloning, isolation, and characterization of replication regions of complex plasmid genomes.

Authors: K Timmis; F Cabello; S N Cohen
Journal: Proc Natl Acad Sci U S A Date: 1975-06 Impact factor: 11.205

6. Endonuclease R-EcoRII restriction of bacteriophage f1 DNA in vitro: ordering of genes V and VII, location of an RNA promotor for gene VIII.

Authors: G F Vovis; K Horiuchi; N D Zinder
Journal: J Virol Date: 1975-09 Impact factor: 5.103