Literature DB >> 6087339

Cell-specified expression of a selectable hybrid gene.

V Episkopou, A J Murphy, A Efstratiadis.   

Abstract

We have inserted into a retroviral vector the structural information encoding the selectable bacterial genes neo and gpt linked, respectively, to the promoters of the herpesvirus thymidine kinase gene and the rat preproinsulin II gene. We have used this recombinant retrovirus construction to transduce insulinoma cell lines that are positive or negative for insulin expression. Selection of the transductants for neo yielded resistant colonies from all of the cell lines, while selection for gpt yielded resistant transductants in relatively high frequency only from insulin-producing cells. The 5' end of the gpt transcripts maps to the authentic preproinsulin capping site of the construction.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6087339      PMCID： PMC391549          DOI： 10.1073/pnas.81.15.4657

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  33 in total

1.  A bacterial clone synthesizing proinsulin.

Authors:  L Villa-Komaroff; A Efstratiadis; S Broome; P Lomedico; R Tizard; S P Naber; W L Chick; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

2.  Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.

Authors:  J Favaloro; R Treisman; R Kamen
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

3.  The inverted repeats of Tn5 are functionally different.

Authors:  S J Rothstein; R A Jorgensen; K Postle; W S Reznikoff
Journal:  Cell       Date:  1980-03       Impact factor: 41.582

4.  Specific labeling of 3' termini with T4 DNA polymerase.

Authors:  M D Challberg; P T Englund
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

5.  Isolation of high-molecular-weight DNA from mammalian cells.

Authors:  M Gross-Bellard; P Oudet; P Chambon
Journal:  Eur J Biochem       Date:  1973-07-02

6.  The structure and evolution of the two nonallelic rat preproinsulin genes.

Authors:  P Lomedico; N Rosenthal; A Efstratidadis; W Gilbert; R Kolodner; R Tizard
Journal:  Cell       Date:  1979-10       Impact factor: 41.582

7.  Expression of a transposable antibiotic resistance element in Saccharomyces.

Authors:  A Jimenez; J Davies
Journal:  Nature       Date:  1980-10-30       Impact factor: 49.962

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor.

Authors:  A F Gazdar; W L Chick; H K Oie; H L Sims; D L King; G C Weir; V Lauris
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

10.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
View more
  27 in total

1.  Insulin gene expression in nonexpressing cells appears to be regulated by multiple distinct negative-acting control elements.

Authors:  S R Cordle; J Whelan; E Henderson; H Masuoka; P A Weil; R Stein
Journal:  Mol Cell Biol       Date:  1991-05       Impact factor: 4.272

2.  Identification of a pancreatic beta-cell insulin gene transcription factor that binds to and appears to activate cell-type-specific expression: its possible relationship to other cellular factors that bind to a common insulin gene sequence.

Authors:  J Whelan; S R Cordle; E Henderson; P A Weil; R Stein
Journal:  Mol Cell Biol       Date:  1990-04       Impact factor: 4.272

3.  A 29-nucleotide DNA segment containing an evolutionarily conserved motif is required in cis for cell-type-restricted repression of the chicken alpha-smooth muscle actin gene core promoter.

Authors:  S L Carroll; D J Bergsma; R J Schwartz
Journal:  Mol Cell Biol       Date:  1988-01       Impact factor: 4.272

4.  Functional analysis of the transcription control region located within the avian retroviral long terminal repeat.

Authors:  B R Cullen; K Raymond; G Ju
Journal:  Mol Cell Biol       Date:  1985-03       Impact factor: 4.272

5.  Generation of helper-free amphotropic retroviruses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene.

Authors:  A D Miller; M F Law; I M Verma
Journal:  Mol Cell Biol       Date:  1985-03       Impact factor: 4.272

6.  Regulated expression of a complete human beta-globin gene encoded by a transmissible retrovirus vector.

Authors:  R D Cone; A Weber-Benarous; D Baorto; R C Mulligan
Journal:  Mol Cell Biol       Date:  1987-02       Impact factor: 4.272

7.  RNA-mediated gene duplication: the rat preproinsulin I gene is a functional retroposon.

Authors:  M B Soares; E Schon; A Henderson; S K Karathanasis; R Cate; S Zeitlin; J Chirgwin; A Efstratiadis
Journal:  Mol Cell Biol       Date:  1985-08       Impact factor: 4.272

8.  Tissue-specific expression of the rat albumin gene: genetic control of its extinction in microcell hybrids.

Authors:  C Petit; J Levilliers; M O Ott; M C Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

9.  Pancreatic beta-cell-type-specific expression of the rat insulin II gene is controlled by positive and negative cellular transcriptional elements.

Authors:  J Whelan; D Poon; P A Weil; R Stein
Journal:  Mol Cell Biol       Date:  1989-08       Impact factor: 4.272

10.  A cell type specific factor recognizes the rat thyroglobulin promoter.

Authors:  A M Musti; V M Ursini; E V Avvedimento; V Zimarino; R Di Lauro
Journal:  Nucleic Acids Res       Date:  1987-10-26       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.