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Literature DB >> 3671079

A cell type specific factor recognizes the rat thyroglobulin promoter.

A M Musti¹, V M Ursini, E V Avvedimento, V Zimarino, R Di Lauro.

Abstract

We have fused a 900 base pair long DNA segment containing the transcriptional start site of the rat thyroglobulin (Tg) gene to the bacterial gene for chloramphenicol acetyltransferase (cat). The fusion gene has been introduced into three different cell lines derived from the rat thyroid gland and into a rat liver cell line. Expression of the fusion gene was detected only in the one thyroid cell line that is able to express the endogenous Tg gene. The minimum DNA sequence required for the cell type specific expression was determined by deletion analysis; it extends 170 nucleotides upstream of the transcription initiation site. The Tg promoter contains a readily detectable binding sites for a factor present in salt extracts of thyroid cell nuclei. This binding site is not recognized by the nuclear extracts of any other cell type that we have tested, suggesting that it may help mediate the cell type specific expression of the Tg gene.

Entities: Gene Species

Mesh：

Substances：
Thyroglobulin

Year: 1987 PMID： 3671079 PMCID： PMC306350 DOI： 10.1093/nar/15.20.8149

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

39 in total

1. Cell- and sequence-specific binding of nuclear proteins to 5'-flanking DNA of the rat growth hormone gene.

Authors: Z S Ye; H H Samuels
Journal: J Biol Chem Date: 1987-05-05 Impact factor: 5.157

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Authors: N K Alton; D Vapnek
Journal: Nature Date: 1979 Dec 20-27 Impact factor: 49.962

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Authors: S P Nissley; P A Short; M M Rechler; J M Podskalny; H G Coon
Journal: Cell Date: 1977-06 Impact factor: 41.582

4. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.

Authors: S D Gillies; S L Morrison; V T Oi; S Tonegawa
Journal: Cell Date: 1983-07 Impact factor: 41.582

5. Construction of recombinant plasmids containing rat thyroglobulin mRNA sequences.

Authors: R Di Lauro; S Obici; A M Acquaviva; C G Alvino
Journal: Gene Date: 1982 Jul-Aug Impact factor: 3.688

6. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.

Authors: C M Gorman; G T Merlino; M C Willingham; I Pastan; B H Howard
Journal: Proc Natl Acad Sci U S A Date: 1982-11 Impact factor: 11.205

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Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

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Authors: C M Gorman; L F Moffat; B H Howard
Journal: Mol Cell Biol Date: 1982-09 Impact factor: 4.272

9. Efficient infection of monkey cells with DNA of simian virus 40.

Authors: L M Sompayrac; K J Danna
Journal: Proc Natl Acad Sci U S A Date: 1981-12 Impact factor: 11.205

10. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors: J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal: Biochemistry Date: 1979-11-27 Impact factor: 3.162

21 in total

1. Expression and function of the homeodomain-containing protein Hex in thyroid cells.

Authors: L Pellizzari; A D'Elia; A Rustighi; G Manfioletti; G Tell; G Damante
Journal: Nucleic Acids Res Date: 2000-07-01 Impact factor: 16.971

2. Pax-8, a paired domain-containing protein, binds to a sequence overlapping the recognition site of a homeodomain and activates transcription from two thyroid-specific promoters.

Authors: M Zannini; H Francis-Lang; D Plachov; R Di Lauro
Journal: Mol Cell Biol Date: 1992-09 Impact factor: 4.272

3. Alpha-cell-specific expression of the glucagon gene is conferred to the glucagon promoter element by the interactions of DNA-binding proteins.

Authors: J Philippe; D J Drucker; W Knepel; L Jepeal; Z Misulovin; J F Habener
Journal: Mol Cell Biol Date: 1988-11 Impact factor: 4.272

4. Neoplastic transformation inactivates specific trans-acting factor(s) required for the expression of the thyroglobulin gene.

Authors: V E Avvedimento; A Musti; A Fusco; M J Bonapace; R Di Lauro
Journal: Proc Natl Acad Sci U S A Date: 1988-03 Impact factor: 11.205

5. Transcriptional regulation of a mouse Clara cell-specific protein (mCC10) gene by the NKx transcription factor family members thyroid transciption factor 1 and cardiac muscle-specific homeobox protein (CSX).

Authors: M K Ray; C Y Chen; R J Schwartz; F J DeMayo
Journal: Mol Cell Biol Date: 1996-05 Impact factor: 4.272

9. Cloning and sequence analysis of TFE, a helix-loop-helix transcription factor able to recognize the thyroglobulin gene promoter in vitro.

Authors: F Javaux; A Donda; G Vassart; D Christophe
Journal: Nucleic Acids Res Date: 1991-03-11 Impact factor: 16.971

10. Thyroid-specific enhancer-binding protein (T/EBP): cDNA cloning, functional characterization, and structural identity with thyroid transcription factor TTF-1.

Authors: K Mizuno; F J Gonzalez; S Kimura
Journal: Mol Cell Biol Date: 1991-10 Impact factor: 4.272