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Literature DB >> 3458217

Tissue-specific expression of the rat albumin gene: genetic control of its extinction in microcell hybrids.

C Petit, J Levilliers, M O Ott, M C Weiss.

Abstract

Numerous studies of cell hybrids have indicated that somatic cells produce negative regulators (extinguishers) that prevent the expression of functions foreign to their own differentiation. Here, we report genetic evidence of such control. In microcell hybrids between well-differentiated rat hepatoma cells and microcells of mouse fibroblast L cells, the extinction of albumin synthesis is directly related to the presence of a single specific chromosome of the mouse fibroblast parent. The expression of several other hepatic functions is not affected. Transfection of these hybrids with a recombinant plasmid, containing a tissue-specific control element of the upstream region of the rat albumin gene linked to coding sequences of the chloramphenicol acetyltransferase gene, reveals that extinction acts on or via this cis-control element.

Entities: Disease Gene Species

Mesh：

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Year: 1986 PMID： 3458217 PMCID： PMC323338 DOI： 10.1073/pnas.83.8.2561

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

43 in total

1. Expression of differentiated functions in hepatoma cell hybrids: selection in glucose-free media of segregated hybrid cells which reexpress gluconeogenic enzymes.

Authors: R Bertolotti
Journal: Somatic Cell Genet Date: 1977-11

2. A selective system for hepatoma cells producing gluconeogenic enzymes.

Authors: R Bertolotti
Journal: Somatic Cell Genet Date: 1977-07

Review 3. Gene expression in somatic cell hybrids.

Authors: R L Davidson
Journal: Annu Rev Genet Date: 1974 Impact factor: 16.830

4. An improved banding technique exemplified in the karyotype analysis of two strains of rat.

Authors: P H Gallimore; C R Richardson
Journal: Chromosoma Date: 1973 Impact factor: 4.316

5. A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.

Authors: H G Coon; M C Weiss
Journal: Proc Natl Acad Sci U S A Date: 1969-03 Impact factor: 11.205

6. Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. I. Evidence for negative control.

Authors: R Davidson; B Ephrussi; K Yamamoto
Journal: J Cell Physiol Date: 1968-10 Impact factor: 6.384

7. A sequential staining technique for the chromosomal analysis of the interspecific mouse/hamster and mouse/human somatic cell hybrids.

Authors: C A Kozak; J B Lawrence; F H Ruddle
Journal: Exp Cell Res Date: 1977-03-01 Impact factor: 3.905

8. Regulation of malanin synthesis in mammalian cells: effect of gene dosage on the expression of differentiation.

Authors: R L Davidson
Journal: Proc Natl Acad Sci U S A Date: 1972-04 Impact factor: 11.205

9. Gene dosage dependence of pigment synthesis in melanoma x fibroblast hybrids (hamster cells-mouse fibroblast-DOPA-oxidase-irradiation).

Authors: C Fougère; F Ruiz; B Ephrussi
Journal: Proc Natl Acad Sci U S A Date: 1972-02 Impact factor: 11.205

10. Expression of differentiated functions in hepatoma cell hybrids: reappearance of tyrosine aminotransferase inducibility after the loss of chromosomes.

Authors: M C Weiss; M Chaplain
Journal: Proc Natl Acad Sci U S A Date: 1971-12 Impact factor: 11.205

28 in total

1. Regulation of hepatitis B virus expression in progenitor and differentiated cell types: evidence for negative transcriptional control in nonpermissive cells.

Authors: M Ott; Q Ma; B Li; S Gagandeep; L E Rogler; S Gupta
Journal: Gene Expr Date: 1999

2. The mouse albumin enhancer contains a negative regulatory element that interacts with a novel DNA-binding protein.

Authors: R S Herbst; E M Boczko; J E Darnell; L E Babiss
Journal: Mol Cell Biol Date: 1990-08 Impact factor: 4.272

3. Extinction of Oct-3/4 gene expression in embryonal carcinoma x fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region.

Authors: E Ben-Shushan; E Pikarsky; A Klar; Y Bergman
Journal: Mol Cell Biol Date: 1993-02 Impact factor: 4.272

Tissue-specific expression of the rat albumin gene: genetic control of its extinction in microcell hybrids.

1. Expression of differentiated functions in hepatoma cell hybrids: selection in glucose-free media of segregated hybrid cells which reexpress gluconeogenic enzymes.

2. A selective system for hepatoma cells producing gluconeogenic enzymes.

Review 3. Gene expression in somatic cell hybrids.

4. An improved banding technique exemplified in the karyotype analysis of two strains of rat.

5. A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.

6. Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. I. Evidence for negative control.

7. A sequential staining technique for the chromosomal analysis of the interspecific mouse/hamster and mouse/human somatic cell hybrids.

8. Regulation of malanin synthesis in mammalian cells: effect of gene dosage on the expression of differentiation.

9. Gene dosage dependence of pigment synthesis in melanoma x fibroblast hybrids (hamster cells-mouse fibroblast-DOPA-oxidase-irradiation).

10. Expression of differentiated functions in hepatoma cell hybrids: reappearance of tyrosine aminotransferase inducibility after the loss of chromosomes.

1. Regulation of hepatitis B virus expression in progenitor and differentiated cell types: evidence for negative transcriptional control in nonpermissive cells.

2. The mouse albumin enhancer contains a negative regulatory element that interacts with a novel DNA-binding protein.

3. Extinction of Oct-3/4 gene expression in embryonal carcinoma x fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region.

4. Coordinate regulation of two genes encoding gluconeogenic enzymes by the trans-dominant locus Tse-1.

5. Hormonal regulation of TSE1-repressed genes: evidence for multiple genetic controls in extinction.

6. Negative regulation contributes to tissue specificity of the immunoglobulin heavy-chain enhancer.

7. Profile of Christine Petit.

8. Extinction of alpha-fetoprotein gene expression in somatic cell hybrids involves cis-acting DNA elements.

9. Multiple hepatic trans-acting factors are required for in vitro transcription of the human alpha-1-antitrypsin gene.

10. Regulation of chimeric phosphoenolpyruvate carboxykinase genes by the trans-dominant locus TSE1.