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Literature DB >> 358198

A bacterial clone synthesizing proinsulin.

L Villa-Komaroff, A Efstratiadis, S Broome, P Lomedico, R Tizard, S P Naber, W L Chick, W Gilbert.

Abstract

We have cloned double-stranded cDNA copies of a rat preproinsulin messenger RNA in Escherichia coli chi1776, using the unique Pst endonuclease site of plasmid pBR322 that lies in the region encoding amino acids 181-182 of penicillinase. This site was reconstructed by inserting the cDNA with an oligo(dG)-oligo(dC) joining procedure. One of the clones expresses a fused protein bearing both insulin and penicillinase antigenic determinants. The DNA sequence of this plasmid shows that the insulin region is read in phase; a stretch of six glycine residues connects the alanine at position 182 of penicillinase to the fourth amino acid, glutamine, of rat proinsulin.

Entities: Chemical Gene Species

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Year: 1978 PMID： 358198 PMCID： PMC392859 DOI： 10.1073/pnas.75.8.3727

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

33 in total

1. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Authors: J Casey; N Davidson
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

2. Immunological screening method to detect specific translation products.

Authors: S Broome; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1978-06 Impact factor: 11.205

3. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

Authors: B M Paterson; B E Roberts; E L Kuff
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

4. Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene.

Authors: S M Tilghman; D C Tiemeier; J G Seidman; B M Peterlin; M Sullivan; J V Maizel; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1978-02 Impact factor: 11.205

5. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

Authors: J G Sutcliffe
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

6. The rabbit beta-globin gene contains a large large insert in the coding sequence.

Authors: A J Jeffreys; R A Flavell
Journal: Cell Date: 1977-12 Impact factor: 41.582

7. Rat insulin genes: construction of plasmids containing the coding sequences.

Authors: A Ullrich; J Shine; J Chirgwin; R Pictet; E Tischer; W J Rutter; H M Goodman
Journal: Science Date: 1977-06-17 Impact factor: 47.728

8. Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.

Authors: R P Ambler; G K Scott
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

9. A transplantable insulinoma in the rat.

Authors: W L Chick; S Warren; R N Chute; A A Like; V Lauris; K C Kitchen
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

10. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors: F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal: Gene Date: 1977 Impact factor: 3.688

167 in total

1. Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

Authors: P J Bassford; T J Silhavy; J R Beckwith
Journal: J Bacteriol Date: 1979-07 Impact factor: 3.490

2. Gentic engineering for practical application.

Authors: W Klingmüller
Journal: Naturwissenschaften Date: 1979-04

3. The rat serum albumin gene: analysis of cloned sequences.

Authors: T D Sargent; J R Wu; J M Sala-Trepat; R B Wallace; A A Reyes; J Bonner
Journal: Proc Natl Acad Sci U S A Date: 1979-07 Impact factor: 11.205

4. Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-beta-lipotropin precursor.

Authors: S Nakanishi; A Inoue; T Kita; S Numa; A C Chang; S N Cohen; J Nunberg; R T Schimke
Journal: Proc Natl Acad Sci U S A Date: 1978-12 Impact factor: 11.205

5. Exploring both sequence detection and restriction endonuclease cleavage kinetics by recognition site via single-molecule microfluidic trapping.

Authors: Weilin Xu; Susan J Muller
Journal: Lab Chip Date: 2010-11-12 Impact factor: 6.799

9. Nucleotide sequence of a cloned cDNA copy of TMV (cowpea strain) RNA, including the assembly origin, the coat protein cistron, and the 3' non-coding region.

Authors: T Meshi; T Ohno; H Iba; Y Okada
Journal: Mol Gen Genet Date: 1981

10. Cloning and expression of human erythropoietin cDNA in Escherichia coli.

Authors: S Lee-Huang
Journal: Proc Natl Acad Sci U S A Date: 1984-05 Impact factor: 11.205