Literature DB >> 271968

DNA sequencing with chain-terminating inhibitors.

F Sanger, S Nicklen, A R Coulson.   

Abstract

A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.

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Year:  1977        PMID： 271968      PMCID： PMC431765          DOI： 10.1073/pnas.74.12.5463

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  Nucleotide and amino acid sequences of gene G of omegaX174.

Authors:  G M Air; F Sanger; A R Coulson
Journal:  J Mol Biol       Date:  1976-12-15       Impact factor: 5.469

2.  In vitro polyoma DNA synthesis: inhibition by 1-beta-d-arabinofuranosyl CTP.

Authors:  T Hunter; B Francke
Journal:  J Virol       Date:  1975-04       Impact factor: 5.103

3.  Nucleotide sequence of bacteriophage phi X174 DNA.

Authors:  F Sanger; G M Air; B G Barrell; N L Brown; A R Coulson; C A Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal:  Nature       Date:  1977-02-24       Impact factor: 49.962

4.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

5.  CV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxyribonucleotide corresponding to the nucleotide sequence 31 to 50.

Authors:  H Büchi; H G Khorana
Journal:  J Mol Biol       Date:  1972-12-28       Impact factor: 5.469

6.  Synthesis of some nucleotides derived from 3'-deoxythymidine.

Authors:  A F Russell; J G Moffatt
Journal:  Biochemistry       Date:  1969-12       Impact factor: 3.162

7.  Enzymatic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotide.

Authors:  M R Atkinson; M P Deutscher; A Kornberg; A F Russell; J G Moffatt
Journal:  Biochemistry       Date:  1969-12       Impact factor: 3.162

8.  DNA synthesis in nucleotide-permeable Escherichia coli cells. The effects of nucleotide analogues on DNA synthesis.

Authors:  K Geider
Journal:  Eur J Biochem       Date:  1972-06-09

9.  Purine nucleosides. XII. The preparation of 2',3'-dideoxyadenosine, 2',5'-dideoxyadenosine, and 2',3',5'-trideoxyadenosine from 2'-deoxyadenosine.

Authors:  M J Robins; J R McCarthy; R K Robins
Journal:  Biochemistry       Date:  1966-01       Impact factor: 3.162

10.  CONVERSION OF MONO- AND OLIGODEOXYRIBONUCLEOTIDES TO 5-TRIPHOSPHATES.

Authors:  D E HOARD; D G OTT
Journal:  J Am Chem Soc       Date:  1965-04-20       Impact factor: 15.419
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  2000 in total

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Authors:  A K Nielsen; S Douthwaite; B Vester
Journal:  RNA       Date:  1999-08       Impact factor: 4.942

2.  Age-related alterations of somatic hypermutation and CDR3 lengths in human Vkappa4-expressing B lymphocytes.

Authors:  D Troutaud; M Drouet; C Decourt; C Le Morvan; M Cogné
Journal:  Immunology       Date:  1999-06       Impact factor: 7.397

3.  Discrimination of multidrug-resistant Mycobacterium tuberculosis IS6110 fingerprint subclusters by rpoB gene mutation analysis.

Authors:  I Portugal; S Maia; J Moniz-Pereira
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

4.  The supporting-cell antigen: a receptor-like protein tyrosine phosphatase expressed in the sensory epithelia of the avian inner ear.

Authors:  R P Kruger; R J Goodyear; P K Legan; M E Warchol; Y Raphael; D A Cotanche; G P Richardson
Journal:  J Neurosci       Date:  1999-06-15       Impact factor: 6.167

5.  Pim kinase expression is induced by LTP stimulation and required for the consolidation of enduring LTP.

Authors:  U Konietzko; G Kauselmann; J Scafidi; U Staubli; H Mikkers; A Berns; M Schweizer; R Waltereit; D Kuhl
Journal:  EMBO J       Date:  1999-06-15       Impact factor: 11.598

6.  Mechanism of repression of the aroP P2 promoter by the TyrR protein of Escherichia coli.

Authors:  J Yang; P Wang; A J Pittard
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

7.  Mutation analysis of the 5' untranslated region of the cold shock cspA mRNA of Escherichia coli.

Authors:  K Yamanaka; M Mitta; M Inouye
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

8.  Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa.

Authors:  T Köhler; S F Epp; L K Curty; J C Pechère
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

9.  Proteophosphoglycans of Leishmania mexicana. Molecular cloning and characterization of the Leishmania mexicana ppg2 gene encoding the proteophosphoglycans aPPG and pPPG2 that are secreted by amastigotes and promastigotes.

Authors:  U Göpfert; N Goehring; C Klein; T Ilg
Journal:  Biochem J       Date:  1999-12-15       Impact factor: 3.857

10.  High-level aminoglycoside resistance in the beta-hemolytic group G Streptococcus isolate BM2721.

Authors:  M Galimand; T Lambert; G Gerbaud; P Courvalin
Journal:  Antimicrob Agents Chemother       Date:  1999-12       Impact factor: 5.191
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