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Literature DB >> 49289

Mammalian chromosome identification in interspecific hybride cells using "Hoechst 33258".

R S Kucherlapati, I Hilwig, A Gropp, F H Ruddle.

Abstract

Treatment of fixed chromosome preparations of mouse-human hybrid cells with the benzimidazol derivative "Hoechst 33258" makes it possible to distinguish between mouse and human chromosomes. Treatment of live cells results in differential condensation of mouse and human centromeric regions. The effects of the dye on a mouse-human translocation and the species specific differences of staining and of condensation are in accordance with the assumption that the action of "H 33258" is specifically dependent on the base composition of the DNA.

Entities: Chemical Species

Mesh：

Substances：

Year: 1975 PMID： 49289

Source DB: PubMed Journal: Humangenetik ISSN： 0018-7348

8 in total

Review 1. The structure and function of chromatin.

Authors: D E Comings
Journal: Adv Hum Genet Date: 1972

2. A somatic cell approach to the genetic analysis of the laboratory mouse.

Authors: C Kozak; E A Nichols; F H Ruddle
Journal: J Exp Zool Date: 1974-02

3. Isolation and characterization of hybrids between mouse and Chinese hamster cell lines.

Authors: H Koyama; I Yatabe; T Ono
Journal: Exp Cell Res Date: 1970-10 Impact factor: 3.905

4. Decondensation of constitutive heterochromatin in L cell chromosomes by a benzimidazole compound ("33258 Hoechst").

Authors: I Hilwig; A Gropp
Journal: Exp Cell Res Date: 1973-10 Impact factor: 3.905

5. Method for locating the centromeres of mouse meiotic chromosomes and its application to T163H and T70H translocations.

Authors: V G Dev; D A Miller; P W Allderdice; O J Miller
Journal: Exp Cell Res Date: 1972-07 Impact factor: 3.905

6. Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochrome.

Authors: I Hilwig; A Gropp
Journal: Exp Cell Res Date: 1972-11 Impact factor: 3.905

7. Identification of Ph1 chromosome and associated translocation in chronic myelogenous leukemia by Hoechst 33258.

Authors: T Raposa; A T Natarajan; I Granberg
Journal: J Natl Cancer Inst Date: 1974-06 Impact factor: 13.506

8. Fluorescent probes of chromosomal DNA structure: three classes of acridines.

Authors: B Weisblum
Journal: Cold Spring Harb Symp Quant Biol Date: 1974

8 in total

19 in total

1. Cytoplasmic inheritance in mammalian tissue culture cells.

Authors: D C Wallace; Y Pollack; C L Bunn; J M Eisenstadt
Journal: In Vitro Date: 1976-11

2. Inhibition of condensation of human Y chromosome by the fluorochrome Hoechst 33258 in a mouse-human cell hybrid.

Authors: M Marcus; A Nattenberg; R Goitein; K Nielsén; A Gropp
Journal: Hum Genet Date: 1979-01-25 Impact factor: 4.132

3. Effect of Hoechst 33258 on Chinese hamster chromosomes.

Authors: A Rocchi; G Prantera; S Pimpinelli; M Di Castro
Journal: Chromosoma Date: 1976-06-30 Impact factor: 4.316

4. Condensation of all human chromosomes in phase G2 and early mitosis can be drastically inhibited by 33258-Hoechst treatment.

Authors: M Marcus; R Goitein; A Gropp
Journal: Hum Genet Date: 1979-09-02 Impact factor: 4.132

5. Genetic control of cobalamin binding in normal and mutant cells: assignment of the gene for 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase to human chromosome 1.

Authors: I S Mellman; P F Lin; F H Ruddle; L E Rosenberg
Journal: Proc Natl Acad Sci U S A Date: 1979-01 Impact factor: 11.205