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Literature DB >> 61845

Effect of Hoechst 33258 on Chinese hamster chromosomes.

A Rocchi, G Prantera, S Pimpinelli, M Di Castro.

Abstract

Cells of the Chinese hamster strain C-125 were treated for different time intervals with H 33258, a bibenzimidazole derivative. The same compound was used to stain fixed cells of the same strain. H 33258 induced in cells in culture specific areas of reduced spiralization on the metaphase chromosomes of some cells. These probably correspond to DNA segments rich in A-T bases interspersed along the chromosomes. Probably H 33258 acts during S period of cell cycle. The banding obtained by staining with H 33258 is similar to that induced by quinacrine dihydrochloride but shows a better resolution.

Entities: Chemical Species

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Year: 1976 PMID： 61845 DOI： 10.1007/BF00293728

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

14 in total

1. The sex chromosomes of the Chinese hamster: constitutive heterochromatin deficient in repetitive DNA sequences.

Authors: F E Arrighi; T C Hsu; S Pathak; H Sawada
Journal: Cytogenet Cell Genet Date: 1974

2. Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA.

Authors: B Weisblum; E Haenssler
Journal: Chromosoma Date: 1974 Impact factor: 4.316

2. Condensation of all human chromosomes in phase G2 and early mitosis can be drastically inhibited by 33258-Hoechst treatment.

Authors: M Marcus; R Goitein; A Gropp
Journal: Hum Genet Date: 1979-09-02 Impact factor: 4.132

3. Quantitative variation of "Mus musculus-like" constitutive heterochromatin and satellite DNA-sequences in the genus Mus.

Authors: S Sen; T Sharma
Journal: Chromosoma Date: 1980 Impact factor: 4.316

4. Characterization of Drosophila heterochromatin. I. Staining and decondensation with Hoechst 33258 and quinacrine.

Authors: M Gatti; S Pimpinelli; G Santini
Journal: Chromosoma Date: 1976-09-24 Impact factor: 4.316

5. The interaction of Hoechst 33258 and BrdU substituted DNA in the formation of sister chromatid exchanges.

Authors: D G Stetka; A V Carrano
Journal: Chromosoma Date: 1977-08-18 Impact factor: 4.316

5 in total

Effect of Hoechst 33258 on Chinese hamster chromosomes.

1. The sex chromosomes of the Chinese hamster: constitutive heterochromatin deficient in repetitive DNA sequences.

2. Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA.

3. Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.

4. Decondensation of constitutive heterochromatin in L cell chromosomes by a benzimidazole compound ("33258 Hoechst").

5. Banding patterns of Chinese hamster chromosomes revealed by new techniques.

6. Chromosomal and nuclear location of mouse satellite DNA in individual cells.

7. Chromosomal localization of mouse satellite DNA.

8. Replication of repetitious DNA and the S period.

9. Evidence for heterogeneity in heterochromatin of Drosophila melanogaster.

10. Mammalian chromosome identification in interspecific hybride cells using "Hoechst 33258".

1. Hoechst 33258 induced uncondensed sites in marsupial chromosomes.

2. Condensation of all human chromosomes in phase G2 and early mitosis can be drastically inhibited by 33258-Hoechst treatment.

3. Quantitative variation of "Mus musculus-like" constitutive heterochromatin and satellite DNA-sequences in the genus Mus.

4. Characterization of Drosophila heterochromatin. I. Staining and decondensation with Hoechst 33258 and quinacrine.

5. The interaction of Hoechst 33258 and BrdU substituted DNA in the formation of sister chromatid exchanges.