Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The use of distamycin A in human lymphocyte cultures.

Literature DB >> 6693124

The use of distamycin A in human lymphocyte cultures.

M Schmid, D A Hungerford, A Poppen, W Engel.

Abstract

The effect of the oligopeptide antibiotic distamycin A on human lymphocyte cultures was examined. Distamycin A specifically inhibits the condensation of the Y heterochromatin and induces a fragile site in the chromosome 16 (band q22) in some individuals. The optimal culture conditions under which an undercondensation of the Y heterochromatin and an induction of the fragile site in 16q22 can be achieved by in vitro treatment of lymphocytes were determined. This also permits the use of distamycin A in routine diagnostics of human chromosomes. The use of this technique in the analysis of translocations involving the Y chromosome is presented. The distamycin A-DNA interaction and the different possible explanations for the distamycin A-induced undercondensations of the Y heterochromatin and fragile sites 16q22 are discussed.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1984 PMID： 6693124 DOI： 10.1007/bf00291563

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

32 in total

1. The importance of being a fragile site.

Authors: F Hecht; B Kaiser-McCaw
Journal: Am J Hum Genet Date: 1979-03 Impact factor: 11.025

2. Heritable fragile sites on human chromosomes II. Distribution, phenotypic effects, and cytogenetics.

Authors: G R Sutherland
Journal: Am J Hum Genet Date: 1979-03 Impact factor: 11.025

3. Decondensation of constitutive heterochromatin in L cell chromosomes by a benzimidazole compound ("33258 Hoechst").

Authors: I Hilwig; A Gropp
Journal: Exp Cell Res Date: 1973-10 Impact factor: 3.905

4. Chromosome structure and function in man. I. Pachytene mapping in the male, improved methods and general discussion of initial results.

Authors: D A Hungerford
Journal: Cytogenetics Date: 1971

5. A new case of Y to X translocation in a female.

Authors: T Hecht; H J Cooke; M Cerrillo; B Meer; G Reck; H Hameister
Journal: Hum Genet Date: 1980 Impact factor: 4.132

6. The fragile site on chromosome 16 (q21q22). Data on four new families.

Authors: F Shabtai; S Bichacho; I Halbrecht
Journal: Hum Genet Date: 1980 Impact factor: 4.132

7. Chromosome structure and function in man. VI. Pachytene chromomere maps of 16, 17 and 18; pachytene as a reference standard for metaphase banding.

Authors: D A Hungerford; A M Hungerford
Journal: Cytogenet Cell Genet Date: 1978

8. An international system for human cytogenetic nomenclature--high-resolution banding (1981). ISCN (1981). Report of the Standing Committee on Human Cytogenetic Nomenclature.

Authors:
Journal: Cytogenet Cell Genet Date: 1981

9. The effects of distamycin A on gorilla-, chimpanzee- and orangutan lymphocyte cultures.

Authors: M Schmid; A Poppen; W Engel
Journal: Cytogenet Cell Genet Date: 1981

10. Epstein-Barr-virus-induced lymphoproliferative disorder converting to fatal Burkitt-like lymphoma in a boy with interferon-inducible chromosomal defect.

Authors: K Thestrup-Pedersen; V Esmann; S Bisballe; J R Jensen; G Pallesen; J Hastrup; M Madsen; K Thorling; M Grazia-Masucci; A K Saemundsen; I Ernberg
Journal: Lancet Date: 1980-11-08 Impact factor: 79.321

8 in total

8. Familial fragile site found at the cancer breakpoint (1)(q32). Inducibility by distamycin A, concomitance with fragile (16)(q22).

Authors: F Shabtai; J Hart; D Klar; I Halbrecht
Journal: Hum Genet Date: 1986-07 Impact factor: 4.132

8 in total

The use of distamycin A in human lymphocyte cultures.

1. The importance of being a fragile site.

2. Heritable fragile sites on human chromosomes II. Distribution, phenotypic effects, and cytogenetics.

3. Decondensation of constitutive heterochromatin in L cell chromosomes by a benzimidazole compound ("33258 Hoechst").

4. Chromosome structure and function in man. I. Pachytene mapping in the male, improved methods and general discussion of initial results.

5. A new case of Y to X translocation in a female.

6. The fragile site on chromosome 16 (q21q22). Data on four new families.

7. Chromosome structure and function in man. VI. Pachytene chromomere maps of 16, 17 and 18; pachytene as a reference standard for metaphase banding.

8. An international system for human cytogenetic nomenclature--high-resolution banding (1981). ISCN (1981). Report of the Standing Committee on Human Cytogenetic Nomenclature.

9. The effects of distamycin A on gorilla-, chimpanzee- and orangutan lymphocyte cultures.

10. Epstein-Barr-virus-induced lymphoproliferative disorder converting to fatal Burkitt-like lymphoma in a boy with interferon-inducible chromosomal defect.

1. "Spontaneous" FRA16B is a hot spot for sister chromatid exchanges.

2. Inhibition of condensation in human chromosomes induced by the thymidine analogue 5-iododeoxyuridine.

3. 5-azacytidine produces differential undercondensation of alpha, beta and classical human satellite DNAs.

4. Different inducibility and possible significance of several concomitant "fragile sites" in two brothers.

5. The fragile site (16) (q22). I. Induction by AT-specific DNA-ligands and population frequency.

6. The fragile site (16)(q22). II. Sister chromatid exchanges.

7. Specific silver staining of experimentally undercondensed chromosome regions.

8. Familial fragile site found at the cancer breakpoint (1)(q32). Inducibility by distamycin A, concomitance with fragile (16)(q22).