Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Inhibition of condensation of human Y chromosome by the fluorochrome Hoechst 33258 in a mouse-human cell hybrid.

Literature DB >> 84782

Inhibition of condensation of human Y chromosome by the fluorochrome Hoechst 33258 in a mouse-human cell hybrid.

M Marcus, A Nattenberg, R Goitein, K Nielsén, A Gropp.

Abstract

The fluorochrome Hoechst 33258 which binds preferentially to A-T base pairs, drastically inhibits the condensation of A-T-rich centromeric heterochromatin regions in mouse cell lines. The condensation of all other regions of these chromosomes is also inhibited to some extent. The human Y chromosome contains a large heterochromatic region, which is also rich in A-T base pairs. This chromosome is not affected by Hoechst 33258 in human leukocyte cell cultures. On the other hand, condensation of the multiple copies of human Y chromosome in the mouse-human cell hybrid RH-28Y-23 is inhibited and the chromosomes appear distorted in Hoechst 33258-treated cells.

Entities: Chemical Species

Mesh：

Substances：

Year: 1979 PMID： 84782 DOI： 10.1007/BF00291921

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

12 in total

1. Human-mouse cell hybrid with human multiple Y chromosomes.

Authors: M Marcus; R Tantravahi; V G Dev; D A Miller; O J Miller
Journal: Nature Date: 1976-07-01 Impact factor: 49.962

2. Lateral asymmetry in the fluorescence of human Y chromosomes stained with 33 258 Hoechst.

Authors: S A Latt; R L Davidson; M S Lin; P S Gerald
Journal: Exp Cell Res Date: 1974-08 Impact factor: 3.905

3. Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA.

Authors: B Weisblum; E Haenssler
Journal: Chromosoma Date: 1974 Impact factor: 4.316

2. Condensation of all human chromosomes in phase G2 and early mitosis can be drastically inhibited by 33258-Hoechst treatment.

Authors: M Marcus; R Goitein; A Gropp
Journal: Hum Genet Date: 1979-09-02 Impact factor: 4.132

3. The use of distamycin A in human lymphocyte cultures.

Authors: M Schmid; D A Hungerford; A Poppen; W Engel
Journal: Hum Genet Date: 1984 Impact factor: 4.132

3 in total

Inhibition of condensation of human Y chromosome by the fluorochrome Hoechst 33258 in a mouse-human cell hybrid.

1. Human-mouse cell hybrid with human multiple Y chromosomes.

2. Lateral asymmetry in the fluorescence of human Y chromosomes stained with 33 258 Hoechst.

3. Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA.

4. Decondensation of constitutive heterochromatin in L cell chromosomes by a benzimidazole compound ("33258 Hoechst").

5. Microfluorometric detection of asymmetry in the centromeric region of mouse chromosomes.

6. Effects of Hoechst 33258 on human leukocytes in vitro.

7. Optical studies of the interaction of 33258 Hoechst with DNA, chromatin, and metaphase chromosomes.

8. The G-banded prophase chromosomes of man.

9. Mechanisms of chromosome banding. VIII. Hoechst 33258-DNA interaction.

10. Mammalian chromosome identification in interspecific hybride cells using "Hoechst 33258".

1. Hoechst 33258 induced uncondensed sites in marsupial chromosomes.

2. Condensation of all human chromosomes in phase G2 and early mitosis can be drastically inhibited by 33258-Hoechst treatment.

3. The use of distamycin A in human lymphocyte cultures.