Literature DB >> 4638799

The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide.

K Brocklehurst, E M Crook, M Kierstan.   

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Year:  1972        PMID： 4638799      PMCID： PMC1173919          DOI： 10.1042/bj1280979

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


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  6 in total

1.  A novel reactivity of papain and a convenient active site titration in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  FEBS Lett       Date:  1970-07-29       Impact factor: 4.124

2.  KINETIC STUDIES OF BROMELAIN CATALYSIS.

Authors:  T INAGAMI; T MURACHI
Journal:  Biochemistry       Date:  1963 Nov-Dec       Impact factor: 3.162

3.  PURIFICATION AND PHYSICAL CHARACTERIZATION OF STEM BROMELAIN.

Authors:  T MURACHI; M YASUI; Y YASUDA
Journal:  Biochemistry       Date:  1964-01       Impact factor: 3.162

4.  The preparation and some properties of bromelain covalently attached to O-(carboxymethyl)-cellulose.

Authors:  C W Wharton; E M Crook; K Brocklehurst
Journal:  Eur J Biochem       Date:  1968-12-05

5.  Evidence for glycoprotein nature of stem bromelain. Isolation of a glycopeptide.

Authors:  T Murachi; A Suzuki; N Takahashi
Journal:  Biochemistry       Date:  1967-12       Impact factor: 3.162

6.  Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1972-06       Impact factor: 3.857
  6 in total
  9 in total

1.  PH-dependence of the steady-state rate of a two-step enzymic reaction.

Authors:  K Brocklehurst; H B Dixon
Journal:  Biochem J       Date:  1976-04-01       Impact factor: 3.857

2.  A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

Authors:  T Stuchbury; M Shipton; R Norris; J P Malthouse; K Brocklehurst; J A Herbert; H Suschitzky
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

3.  Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.

Authors:  M Shipton; K Brochlehurst
Journal:  Biochem J       Date:  1978-05-01       Impact factor: 3.857

4.  Covalent chromatography. Preparation of fully active papain from dried papaya latex.

Authors:  K Brocklehurst; J Carlsson; M P Kierstan; E M Crook
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

5.  4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain.

Authors:  M Shipton; T Stuchbury; K Brocklehurst
Journal:  Biochem J       Date:  1976-11       Impact factor: 3.857

6.  Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelain- and papain-catalysed hydrolyses.

Authors:  C W Wharton; A Cornish-Bowden; K Brocklehurst; E M Crook
Journal:  Biochem J       Date:  1974-08       Impact factor: 3.857

7.  The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester.

Authors:  C W Wharton
Journal:  Biochem J       Date:  1974-12       Impact factor: 3.857

8.  Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.

Authors:  J Carlsson; M P Kierstan; K Brocklehurst
Journal:  Biochem J       Date:  1974-04       Impact factor: 3.857

9.  Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active-centre properties that differ from those of papain by using 2,2'-dipyridyl disulphide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Use of two-protonic-state electrophiles in the identification of catalytic-site thiol groups.

Authors:  B S Baines; K Brocklehurst
Journal:  Biochem J       Date:  1982-07-01       Impact factor: 3.857
  9 in total

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