Literature DB >> 11947645

A novel reactivity of papain and a convenient active site titration in the presence of other thiols.

K Brocklehurst1, G Little.   

Abstract

Entities:  

Year:  1970        PMID： 11947645     DOI： 10.1016/0014-5793(70)80327-1

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


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  20 in total

1.  Location of a constriction in the lumen of a transmembrane pore by targeted covalent attachment of polymer molecules.

Authors:  L Movileanu; S Cheley; S Howorka; O Braha; H Bayley
Journal:  J Gen Physiol       Date:  2001-03       Impact factor: 4.086

2.  A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

Authors:  T Stuchbury; M Shipton; R Norris; J P Malthouse; K Brocklehurst; J A Herbert; H Suschitzky
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

3.  The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.

Authors:  D Kowlessur; M O'Driscoll; C M Topham; W Templeton; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

4.  Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.

Authors:  M Shipton; K Brochlehurst
Journal:  Biochem J       Date:  1978-05-01       Impact factor: 3.857

5.  Covalent chromatography. Preparation of fully active papain from dried papaya latex.

Authors:  K Brocklehurst; J Carlsson; M P Kierstan; E M Crook
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

6.  The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide.

Authors:  K Brocklehurst; E M Crook; M Kierstan
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

7.  Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.

Authors:  J Carlsson; M P Kierstan; K Brocklehurst
Journal:  Biochem J       Date:  1974-04       Impact factor: 3.857

8.  Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857

9.  Investigation of the active site of papain with fluorescent probes.

Authors:  G Allen; G Lowe
Journal:  Biochem J       Date:  1973-08       Impact factor: 3.857

10.  Preparation of cathepsins B and H by covalent chromatography and characterization of their catalytic sites by reaction with a thiol-specific two-protonic-state reactivity probe. Kinetic study of cathepsins B and H extending into alkaline media and a rapid spectroscopic titration of cathepsin H at pH 3-4.

Authors:  F Willenbrock; K Brocklehurst
Journal:  Biochem J       Date:  1985-04-15       Impact factor: 3.857
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