Literature DB >> 5084800

Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.

K Brocklehurst, G Little.   

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Year:  1972        PMID： 5084800      PMCID： PMC1173786          DOI： 10.1042/bj1280471

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


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  5 in total

1.  A novel reactivity of papain and a convenient active site titration in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  FEBS Lett       Date:  1970-07-29       Impact factor: 4.124

2.  KINETICS OF PAPAIN-CATALYZED HYDROLYSIS OF ALPHA-N-BENZOYL-L-ARGININE ETHYL ESTER AND ALPHA-N-BENZOYL-L-ARGININAMIDE.

Authors:  J R WHITAKER; M L BENDER
Journal:  J Am Chem Soc       Date:  1965-06-20       Impact factor: 15.419

3.  IV. Cysteine proteinases. The structure of the papain molecule.

Authors:  J Drenth; J N Jansonius; R Koekoek; L A Sluyterman; B G Wolthers
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1970-02-12       Impact factor: 6.237

4.  Evidence for histidine in the active site of papain.

Authors:  S S Husain; G Lowe
Journal:  Biochem J       Date:  1968-08       Impact factor: 3.857

5.  Kinetics of the reversible reaction of papain with 5,5'-dithiobis-(2-nitrobenzoate) dianion: evidence for nucleophilic reactivity in the un-ionized thiol group of cysteine-25 and for general acid catalysis by histidine-159 of the reaction of the 5-mercapto-2-nitrobenzoate dianion with the papain-5-mercapto-2-nitrobenzoate mixed disulphide.

Authors:  G Little; K Brocklehurst
Journal:  Biochem J       Date:  1972-06       Impact factor: 3.857
  5 in total
  27 in total

1.  A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

Authors:  T Stuchbury; M Shipton; R Norris; J P Malthouse; K Brocklehurst; J A Herbert; H Suschitzky
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

2.  The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.

Authors:  D Kowlessur; M O'Driscoll; C M Topham; W Templeton; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

3.  Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain.

Authors:  D Kowlessur; C M Topham; E W Thomas; M O'Driscoll; W Templeton; K Brocklehurst
Journal:  Biochem J       Date:  1989-03-15       Impact factor: 3.857

4.  Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.

Authors:  M Shipton; K Brochlehurst
Journal:  Biochem J       Date:  1978-05-01       Impact factor: 3.857

5.  Covalent chromatography. Preparation of fully active papain from dried papaya latex.

Authors:  K Brocklehurst; J Carlsson; M P Kierstan; E M Crook
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

6.  Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes.

Authors:  K Brocklehurst; J A Herbert; R Norris; H Suschitzky
Journal:  Biochem J       Date:  1979-11-01       Impact factor: 3.857

7.  A convenient spectrophotometric assay for the determination of L-ergothioneine in blood.

Authors:  J Carlsson; M P Kierstan; K Brocklehurst
Journal:  Biochem J       Date:  1974-04       Impact factor: 3.857

8.  Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.

Authors:  J Carlsson; M P Kierstan; K Brocklehurst
Journal:  Biochem J       Date:  1974-04       Impact factor: 3.857

9.  Oxidant resistance in a yeast mutant deficient in the Sit4 phosphatase.

Authors:  H Reynaldo López-Mirabal; Jakob R Winther; Morten C Kielland-Brandt
Journal:  Curr Genet       Date:  2008-03-21       Impact factor: 3.886

10.  Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857
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