Literature DB >> 5701971

The preparation and some properties of bromelain covalently attached to O-(carboxymethyl)-cellulose.

C W Wharton, E M Crook, K Brocklehurst.   

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Year:  1968        PMID: 5701971     DOI: 10.1111/j.1432-1033.1968.tb00482.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN: 0014-2956


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  8 in total

1.  A general kinetic approach to investigation of active-site availability in macromolecular catalysts.

Authors:  M Resmini; S Gul; S Carter; S Sonkaria; C M Topham; G Gallacher; K Brocklehurst
Journal:  Biochem J       Date:  2000-02-15       Impact factor: 3.857

Review 2.  Enzyme stabilization: state of the art.

Authors:  L Gianfreda; M R Scarfi
Journal:  Mol Cell Biochem       Date:  1991-02-02       Impact factor: 3.396

3.  The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide.

Authors:  K Brocklehurst; E M Crook; M Kierstan
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

4.  Ionic control of immobilized enzymes. Kinetics of acid phosphatase bound to plant cell walls.

Authors:  J Ricard; G Noat; M Crasnier; D Job
Journal:  Biochem J       Date:  1981-05-01       Impact factor: 3.857

5.  Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelain- and papain-catalysed hydrolyses.

Authors:  C W Wharton; A Cornish-Bowden; K Brocklehurst; E M Crook
Journal:  Biochem J       Date:  1974-08       Impact factor: 3.857

6.  The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester.

Authors:  C W Wharton
Journal:  Biochem J       Date:  1974-12       Impact factor: 3.857

7.  Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857

8.  Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.

Authors:  M P Thomas; C M Topham; D Kowlessur; G W Mellor; E W Thomas; D Whitford; K Brocklehurst
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857

  8 in total

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