Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose.

Literature DB >> 4138457

Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose.

Abstract

Polycytidylic acid [poly(rC)] covalently linked to cyanogen bromide-activated agarose is an effective affinity matrix for the RNA-dependent DNA polymerase from avian myeloblastosis virus. Poly(rC)-agarose is capable of binding large quantities of avian myeloblastosis DNA polymerase, which is then eluted by using a linear KCl gradient of increasing concentration. The DNA polymerase isolated from crude, detergent-disrupted virions by a single pass through columns of poly(rC)-agarose appears nearly homogeneous (approximately 90% pure) as determined by sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis. Complete recovery of input enzymatic activity was obtained. Results suggest that polyribonucleotide columns may provide a high-yield, rapid method for the purification of oncornaviral DNA polymerase.

Entities: Chemical Species

Mesh：

Substances：

Year: 1974 PMID： 4138457 PMCID： PMC355591

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

21 in total

1. Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.

Authors: P Setlow; D Brutlag; A Kornberg
Journal: J Biol Chem Date: 1972-01-10 Impact factor: 5.157

2. Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.

Authors: D M Livingston; E M Scolnick; W P Parks; G T Todaro
Journal: Proc Natl Acad Sci U S A Date: 1972-02 Impact factor: 11.205

3. Purification of the DNA polymerase of avian myeloblastosis virus.

Authors: D L Kacian; K F Watson; A Burny; S Spiegelman
Journal: Biochim Biophys Acta Date: 1971-09-24

Review 4. Affinity chromatography.

Authors: P Cuatrecasas
Journal: Annu Rev Biochem Date: 1971 Impact factor: 23.643

2. Sequence of 1060 3'-terminal nucleotides of poliovirus RNA as determined by a modification of the dideoxynucleotide method.

Authors: N Kitamura; E Wimmer
Journal: Proc Natl Acad Sci U S A Date: 1980-06 Impact factor: 11.205

9. Resolution and characterization of intracytoplasmic forms of reverse transcriptase from Rauscher leukemia virus-producing cells.

Authors: S L Marcus
Journal: J Virol Date: 1978-04 Impact factor: 5.103

10. Multiple RNase H activities in mammalian type C retravirus lysates.

Authors: G F Gerard
Journal: J Virol Date: 1978-04 Impact factor: 5.103

10 in total

Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose.

1. Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.

2. Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.

3. Purification of the DNA polymerase of avian myeloblastosis virus.

Review 4. Affinity chromatography.

5. Viral and cellular DNA polymerase: comparison of activities with synthetic and natural RNA templates.

6. An oligonucleotide affinity column for RNA-dependent DNA polymerase from RNA tumor viruses.

7. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

9. Covalent attachment of nucleic acids to agarose for affinity chromatography.

10. Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases.

1. Affinity chromatography of viral DNA polymerases on pyran-sepharose.

2. Sequence of 1060 3'-terminal nucleotides of poliovirus RNA as determined by a modification of the dideoxynucleotide method.

3. RNA-directed DNA polymerase from particles released by normal goose cells.

4. An RNA-dependent DNA polymerase, different from the known viral reverse transcriptases, in the chicken system.

5. Specific inhibition of DNA polymerase-associated RNase H by DNA.

6. Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase.

7. Replication of semliki forest virus: polyadenylate in plus-strand RNA and polyuridylate in minus-strand RNA.

8. Purification of the alpha subunit of avian myeloblastosis virus DNA polymerase by polyuridylic acid-sepharose.

9. Resolution and characterization of intracytoplasmic forms of reverse transcriptase from Rauscher leukemia virus-producing cells.

10. Multiple RNase H activities in mammalian type C retravirus lysates.