Literature DB >> 4506781

An oligonucleotide affinity column for RNA-dependent DNA polymerase from RNA tumor viruses.

B I Gerwin, J B Milstien.   

Abstract

Columns of (dT)(12-18)-cellulose provide a one-step enrichment procedure for RNA-dependent DNA polymerase. The enzyme of the virus from RD-114 cells, as well as that from Rauscher murine leukemia virus, have been purified in this way. The preference of viral as compared to cellular DNA polymerases for (dT)(12-18) as a primer is reflected in the fact that the DNA polymerases of uninfected cells do not bind to this column. Viral enzymes have been purified and identified from crude cellular extracts.

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Year:  1972        PMID： 4506781      PMCID： PMC426997          DOI： 10.1073/pnas.69.9.2599

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  RNA-dependent DNA polymerase activity of RNA tumor viruses. I. Directing influence of DNA in the reaction.

Authors:  J Hurwitz; J P Leis
Journal:  J Virol       Date:  1972-01       Impact factor: 5.103

2.  Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.

Authors:  D M Livingston; E M Scolnick; W P Parks; G T Todaro
Journal:  Proc Natl Acad Sci U S A       Date:  1972-02       Impact factor: 11.205

3.  Immunological characterization of primate C-type virus reverse transcriptases.

Authors:  E M Scolnick; W P Parks; G J Todaro; S A Aaronson
Journal:  Nat New Biol       Date:  1972-01-12

4.  RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.

Authors:  E Scolnick; E Rands; S A Aaronson; G J Todaro
Journal:  Proc Natl Acad Sci U S A       Date:  1970-12       Impact factor: 11.205

5.  Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures.

Authors:  R H Bassin; N Tuttle; P J Fischinger
Journal:  Int J Cancer       Date:  1970-07-15       Impact factor: 7.396

Review 6.  Affinity chromatography.

Authors:  P Cuatrecasas
Journal:  Annu Rev Biochem       Date:  1971       Impact factor: 23.643

7.  Separation of murine cellular and murine leukaemia virus DNA polymerases.

Authors:  J Ross; E M Scolnick; G J Todaro; S A Aaronson
Journal:  Nat New Biol       Date:  1971-06-09

8.  Isolation and identification of a helper virus found in the Moloney sarcoma-leukemia virus complex.

Authors:  P J Fischinger; C O Moore; T E O'Connor
Journal:  J Natl Cancer Inst       Date:  1969-04       Impact factor: 13.506

9.  Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses.

Authors:  D Baltimore; D Smoler
Journal:  Proc Natl Acad Sci U S A       Date:  1971-07       Impact factor: 11.205

10.  Disseminated rhabdomyosarcomas formed in kittens by cultured human rhabdomyosarcoma cells.

Authors:  R M McAllister; W A Nelson-Rees; E Y Johnson; R W Rongey; M B Gardner
Journal:  J Natl Cancer Inst       Date:  1971-09       Impact factor: 13.506
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  20 in total

1.  Effect of UV Light on RNA-Directed DNA Polymerase Activity of Murine Oncornaviruses.

Authors:  G G Lovinger; H P Ling; R V Gilden; M Hatanaka
Journal:  J Virol       Date:  1975-05       Impact factor: 5.103

2.  Affinity chromatography of viral DNA polymerases on pyran-sepharose.

Authors:  J G Chirikjian; L Rye; T S Papas
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

3.  Swan mice: autoimmune mice without xenotropic type C virus production.

Authors:  C Blaineau; S Gisslebrecht; D Piatier; M A Hurot; J P Levy
Journal:  Clin Exp Immunol       Date:  1978-02       Impact factor: 4.330

4.  Murine leukemia virus pol gene products: analysis with antisera generated against reverse transcriptase and endonuclease fusion proteins expressed in Escherichia coli.

Authors:  S C Hu; D L Court; M Zweig; J G Levin
Journal:  J Virol       Date:  1986-10       Impact factor: 5.103

5.  Functional organization of the murine leukemia virus reverse transcriptase: characterization of a bacterially expressed AKR DNA polymerase deficient in RNase H activity.

Authors:  J G Levin; R J Crouch; K Post; S C Hu; D McKelvin; M Zweig; D L Court; B I Gerwin
Journal:  J Virol       Date:  1988-11       Impact factor: 5.103

6.  DNA polymerase of murine sarcoma-leukemia virus: lack of detectable RNase H and low activity with viral RNA and natural DNA templates.

Authors:  L H Wang; P H Duesberg
Journal:  J Virol       Date:  1973-12       Impact factor: 5.103

7.  An oligodeoxynucleotide affinity column for the isolation of sequence specific DNA binding proteins.

Authors:  R Blanks; L W McLaughlin
Journal:  Nucleic Acids Res       Date:  1988-11-11       Impact factor: 16.971

8.  Macromolecular requirements for abrogation of Fv-1 restriction by murine leukemia viruses.

Authors:  R H Bassin; B I Gerwin; J G Levin; G Duran-Troise; B M Benjers; A Rein
Journal:  J Virol       Date:  1980-08       Impact factor: 5.103

9.  Detection and isolation of a new DNA polymerase from human breast tumor cell line HBT-3 by (dT)12-18-cellulose chromatography.

Authors:  B I Gerwin; R H Bassin
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205

10.  Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose.

Authors:  S L Marcus; M J Modak; L F Cavalieri
Journal:  J Virol       Date:  1974-10       Impact factor: 5.103
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