Literature DB >> 4333983

Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.

D M Livingston, E M Scolnick, W P Parks, G T Todaro.   

Abstract

A solid phase immunoadsorbent specific for RNA-dependent DNA polymerase from murine and feline RNA tumor viruses has been prepared. The enzymes from murine and feline virus but not from avian virus bind to columns of this material. Bound enzymes can be eluted in active form. This method has permitted selective purification of viral enzyme from crude extracts of virus-transformed cells, since the immunoadsorbent has no affinity for cellular DNA polymerases.

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Year:  1972        PMID： 4333983      PMCID： PMC426465          DOI： 10.1073/pnas.69.2.393

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  10 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

Review 2.  Affinity chromatography.

Authors:  P Cuatrecasas
Journal:  Annu Rev Biochem       Date:  1971       Impact factor: 23.643

3.  Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells.

Authors:  G J Todaro; S A Aaronson
Journal:  Virology       Date:  1969-05       Impact factor: 3.616

4.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

5.  Selective enzyme purification by affinity chromatography.

Authors:  P Cuatrecasas; M Wilchek; C B Anfinsen
Journal:  Proc Natl Acad Sci U S A       Date:  1968-10       Impact factor: 11.205

6.  A general method for the specific isolation of peptides containing modified residues, using insoluble antibody columns.

Authors:  M Wilchek; V Bocchini; M Becker; D Givol
Journal:  Biochemistry       Date:  1971-07-20       Impact factor: 3.162

7.  Separation of murine cellular and murine leukaemia virus DNA polymerases.

Authors:  J Ross; E M Scolnick; G J Todaro; S A Aaronson
Journal:  Nat New Biol       Date:  1971-06-09

8.  The activation of mutant beta-galactosidase by specific antibodies. Purification of eleven antibody activatable mutant proteins and their subunits on sepharose immunosorbents. Determination of the molecular weights by sedimentation analysis and acrylamide gel electrophoresis.

Authors:  F Melchers; W Messer
Journal:  Eur J Biochem       Date:  1970-12

9.  A general method for the isolation of labelled peptides from affinity-labelled proteins.

Authors:  D Givol; Y Weinstein; M Gorecki; M Wilchek
Journal:  Biochem Biophys Res Commun       Date:  1970-02-20       Impact factor: 3.575

10.  Antibody to the RNA-dependent DNA polymerase of mammalian C-type RNA tumor viruses.

Authors:  S A Aaronson; W P Parks; E M Scolnick; G J Todaro
Journal:  Proc Natl Acad Sci U S A       Date:  1971-05       Impact factor: 11.205
  10 in total
  5 in total

1.  Affinity chromatography of viral DNA polymerases on pyran-sepharose.

Authors:  J G Chirikjian; L Rye; T S Papas
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

2.  An oligonucleotide affinity column for RNA-dependent DNA polymerase from RNA tumor viruses.

Authors:  B I Gerwin; J B Milstien
Journal:  Proc Natl Acad Sci U S A       Date:  1972-09       Impact factor: 11.205

3.  Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose.

Authors:  S L Marcus; M J Modak; L F Cavalieri
Journal:  J Virol       Date:  1974-10       Impact factor: 5.103

4.  Interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type C viruses.

Authors:  C J Sherr; L A Fedele; R E Benveniste; G J Todaro
Journal:  J Virol       Date:  1975-06       Impact factor: 5.103

5.  Radioimmunoassay of mammalian type C viral proteins. 3. Detection of viral antigen in normal murine cells and tissues.

Authors:  W P Parks; D M Livingston; G J Todaro; R E Benveniste; E M Scolnick
Journal:  J Exp Med       Date:  1973-03-01       Impact factor: 14.307
  5 in total

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