Literature DB >> 60740

Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase.

S L Marcus, M J Modak.   

Abstract

The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations were found to vary by as much as 10-fold depending upon the template-primer used to direct synthesis. Addition of KCl to reaction mixtures containing optimal divalent cation concentrations produced stimulation or inhibition of DNA synthesis which was also template-specific. DNA synthesis on the modified template poly (2'-0-methylcytidylate) was uniquely stimulated by combinations of divalent cations. With Mg++ as divalent cation, deviations from classical Michaelis-Menten kinetics of substrate saturation were observed with all template-primers tested.

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Year:  1976        PMID： 60740      PMCID： PMC343006          DOI： 10.1093/nar/3.6.1473

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

1.  Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.

Authors:  H V APOSHIAN; A KORNBERG
Journal:  J Biol Chem       Date:  1962-02       Impact factor: 5.157

2.  RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.

Authors:  E Scolnick; E Rands; S A Aaronson; G J Todaro
Journal:  Proc Natl Acad Sci U S A       Date:  1970-12       Impact factor: 11.205

3.  Poly(2'-O-methylcytidylate)-oligodeoxyguanylate as a template for the ribonucleic acid directed deoxyribonucleic acid polymerase in ribonucleic acid tumor virus particles and a specific probe for the ribonucleic acid directed enzyme in transformed murine cells.

Authors:  G F Gerard; F Rottman; M Green
Journal:  Biochemistry       Date:  1974-04-09       Impact factor: 3.162

Review 4.  RNA-directed DNA polymerase--properties and functions in oncogenic RNA viruses and cells.

Authors:  M Green; G F Gerard
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1974

5.  Comparative biochemical properties of RNA-directed DNA polymerases from Rauscher murine leukemia virus and avian myeloblastosis virus.

Authors:  L C Waters; W K Yang
Journal:  Cancer Res       Date:  1974-10       Impact factor: 12.701

6.  Comparative ability of RNA and DNA to prime DNA synthesis in vitro: role of sequence, sugar, and structure of template-primer.

Authors:  T M Tamblyn; R D Wells
Journal:  Biochemistry       Date:  1975-04-08       Impact factor: 3.162

7.  Avian myeloblastosis virus DNA polymerase. Kinetic studies on the incorporation of noncomplementary nucleotides.

Authors:  N Battula; D Dube; L A Loeb
Journal:  J Biol Chem       Date:  1975-11-10       Impact factor: 5.157

8.  Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose.

Authors:  S L Marcus; M J Modak; L F Cavalieri
Journal:  J Virol       Date:  1974-10       Impact factor: 5.103

9.  RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus: a zinc metalloenzyme.

Authors:  D S Auld; H Kawaguchi; D M Livingston; B L Vallee
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

10.  Evidence for allosterism in in vitro DNA synthesis on RNA templates.

Authors:  L F Cavalieri; M J Modak; S L Marcus
Journal:  Proc Natl Acad Sci U S A       Date:  1974-03       Impact factor: 11.205
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  6 in total

1.  Reverse transcriptase-associated RNase H activity. II. Inhibition by natural and synthetic RNA.

Authors:  S L Marcus; S W Smith; M J Modak
Journal:  J Virol       Date:  1978-09       Impact factor: 5.103

2.  Specific inhibition of DNA polymerase-associated RNase H by DNA.

Authors:  M J Modak; S L Marcus
Journal:  J Virol       Date:  1977-04       Impact factor: 5.103

3.  DNA endonucleases associated with the avian myeloblastosis virus DNA polymerase.

Authors:  K P Samuel; T S Papas; J G Chirikjian
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

4.  On the early emergence of reverse transcription: theoretical basis and experimental evidence.

Authors:  A Lazcano; V Valverde; G Hernández; P Gariglio; G E Fox; J Oró
Journal:  J Mol Evol       Date:  1992-12       Impact factor: 2.395

5.  The effect of magnesium and manganese ions on the structure and template activity for reverse transcriptase of polyribocytidylate and its 2'-0-methyl derivative.

Authors:  N C Vamvakopoulos; J N Vournakis; S L Marcus
Journal:  Nucleic Acids Res       Date:  1977-10       Impact factor: 16.971

6.  Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification.

Authors:  Takahiro Tougan; Daisuke Okuzaki; Hiroshi Nojima
Journal:  Nucleic Acids Res       Date:  2008-07-04       Impact factor: 16.971
  6 in total

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