Literature DB >> 165485

Affinity chromatography of viral DNA polymerases on pyran-sepharose.

J G Chirikjian, L Rye, T S Papas.   

Abstract

Pyran covalently linked to cyanogen bromide-activated Sepharose has been shown to be an effective affinity matrix for several viral DNA polymerases. Differential salt elution of viral compared with cellular polymerases, as well as substrate elution, suggests the affinity nature for the matrix. Unlike some other affinity systems described, pyran-Sepharose is totally resistant to nuclease digestion and is stable at 4 degrees for several months. DNA polymerases isolated from several viruses by detergent treatment were recovered in good yield. Analysis of iodinated proteins by sodium dodecyl sulfate-gel electrophoresis revealed that the DNA polymerase of avian myeloblastosis virus found in crude preparations of the virus could be purified nearly to homogeneity by a single passage through the column. These results suggest that pyran-Sepharose is an effective affinity column that is potentially adaptable as part of a general purification procedure for viral DNA polymerases.

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Year:  1975        PMID： 165485      PMCID： PMC432482          DOI： 10.1073/pnas.72.3.1142

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  25 in total

1.  Specific inhibition of DNA-polymerases from RNA tumor viruses by some new daunomycin derivatives.

Authors:  P Chandra; F Zunino; A Götz; D Gericke; R Thorbeck; A Di Marco
Journal:  FEBS Lett       Date:  1972-04-01       Impact factor: 4.124

2.  ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.

Authors:  C C RICHARDSON; C L SCHILDKRAUT; H V APOSHIAN; A KORNBERG
Journal:  J Biol Chem       Date:  1964-01       Impact factor: 5.157

3.  Affinity elution as a purification method for aminoacyl-tRNA synthetases.

Authors:  F von der Haar
Journal:  Eur J Biochem       Date:  1973-04-02

4.  Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.

Authors:  D M Livingston; E M Scolnick; W P Parks; G T Todaro
Journal:  Proc Natl Acad Sci U S A       Date:  1972-02       Impact factor: 11.205

5.  Viral DNA-dependent DNA polymerase and the properties of thymidine labelled material in virions of an oncogenic RNA virus.

Authors:  J Ríman; G S Beaudreau
Journal:  Nature       Date:  1970-10-31       Impact factor: 49.962

Review 6.  RNA-directed DNA polymerase--properties and functions in oncogenic RNA viruses and cells.

Authors:  M Green; G F Gerard
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1974

7.  Inhibition of RNA-directed DNA polymerase from Rauscher leukemia virus by the 5'-triphosphate of cytosine arabinoside.

Authors:  F W Tuominen; F T Kenney
Journal:  Biochem Biophys Res Commun       Date:  1972-09-26       Impact factor: 3.575

8.  Rifamycin derivatives strongly inhibiting RNA leads to DNA polymerase (reverse transcriptase) of murine sarcoma viruses.

Authors:  C Gurgo; R Ray; M Green
Journal:  J Natl Cancer Inst       Date:  1972-07       Impact factor: 13.506

9.  Inhibition of RNA-dependent DNA polymerase of Rous sarcoma virus by thiosemicarbazones and several cations.

Authors:  W Levinson; A Faras; B Woodson; J Jackson; J M Bishop
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205

10.  Inhibition of AMV DNA polymerase by polyriboadenylic acid containing epsilon-adenosine residues.

Authors:  J G Chirikjian; T S Papas
Journal:  Biochem Biophys Res Commun       Date:  1974-07-24       Impact factor: 3.575
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  5 in total

1.  Template specific inhibitor of mammalian DNA polymerases.

Authors:  J Pitha; S H Wilson
Journal:  Nucleic Acids Res       Date:  1976-03       Impact factor: 16.971

2.  tRNATrp (bovine) binding to the reverse transcriptase of avian myeloblastosis virus and function as a heterologous primer.

Authors:  B M Baroudy; M Fournier; J Labouesse; T S Papas; J G Chirikjian
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205

3.  Mechanism of release of active alpha subunit from dimeric alpha beta avian myeloblastosis virus DNA polymerase.

Authors:  T S Papas; D J Marciani; K Samuel; J G Chirikjian
Journal:  J Virol       Date:  1976-06       Impact factor: 5.103

4.  Biospecific fractionation matrices for sequence specific endonucleases.

Authors:  J George; J G Chirikjian
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

5.  Structural requirements for binding of bovine tRNATrp with avian myeloblastosis virus DNA polymerase.

Authors:  B M Baroudy; J G Chirikjian
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971
  5 in total

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