Literature DB >> 3737406

Synthesis of DNA via deoxynucleoside H-phosphonate intermediates.

B C Froehler, P G Ng, M D Matteucci.   

Abstract

Deoxynucleoside H-phosphonates are used in the chemical synthesis of deoxyoligonucleotides up to 107 bases in length. The biological activity of the synthetic DNA is assessed by cloning into M13 and sequencing. An improved synthesis of protected deoxynucleoside H-phosphonates is also described.

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Year:  1986        PMID： 3737406      PMCID： PMC311548          DOI： 10.1093/nar/14.13.5399

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  4 in total

1.  Syringe method for stepwise chemical synthesis of oligonucleotides.

Authors:  T Tanaka; R L Letsinger
Journal:  Nucleic Acids Res       Date:  1982-05-25       Impact factor: 16.971

2.  Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides.

Authors:  R T Pon; M J Damha; K K Ogilvie
Journal:  Nucleic Acids Res       Date:  1985-09-25       Impact factor: 16.971

3.  Dialkylformamidines: depurination resistant N6-protecting group for deoxyadenosine.

Authors:  B C Froehler; M D Matteucci
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971

4.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
  4 in total
  51 in total

1.  Antiproliferative effects of a c-myc antisense oligonucleotide on human arterial smooth muscle cells.

Authors:  M Ebbecke; C Unterberg; A Buchwald; S Stöhr; V Wiegand
Journal:  Basic Res Cardiol       Date:  1992 Nov-Dec       Impact factor: 17.165

2.  7-Deaza-2'-deoxyadenosine and 3-deaza-2'-deoxyadenosine replacing dA within d(A6)-tracts: differential bending at 3'- and 5'-junctions of d(A6).d(T6) and B-DNA.

Authors:  F Seela; T Grein
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

3.  Modified deoxyoligonucleotides stable to exonuclease degradation in serum.

Authors:  J P Shaw; K Kent; J Bird; J Fishback; B Froehler
Journal:  Nucleic Acids Res       Date:  1991-02-25       Impact factor: 16.971

4.  Template-primer analogs as substrates for DNA polymerase.

Authors:  T R Webb; P Jhurani; P G Ng
Journal:  Nucleic Acids Res       Date:  1987-05-26       Impact factor: 16.971

5.  Oligonucleotide-mediated triple helix formation using an N3-protonated deoxycytidine analog exhibiting pH-independent binding within the physiological range.

Authors:  S H Krawczyk; J F Milligan; S Wadwani; C Moulds; B C Froehler; M D Matteucci
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

6.  A spectroscopic and calorimetric study of the melting behaviors of a "bent" and a "normal" DNA duplex: [d(GA4T4C)]2 versus [d(GT4A4C)]2.

Authors:  Y W Park; K J Breslauer
Journal:  Proc Natl Acad Sci U S A       Date:  1991-02-15       Impact factor: 11.205

7.  Phosphorus-based combinatorial libraries: use of amino acid derivatives as synthons.

Authors:  R Fathi; R Patel; A F Cook
Journal:  Mol Divers       Date:  1997       Impact factor: 2.943

8.  Cleavage of single stranded oligonucleotides by EcoRI restriction endonuclease.

Authors:  N Bischofberger; P G Ng; T R Webb; M D Matteucci
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971

9.  1-Deazaadenosine: synthesis and activity of base-modified hammerhead ribozymes.

Authors:  F Seela; H Debelak; N Usman; A Burgin; L Beigelman
Journal:  Nucleic Acids Res       Date:  1998-02-15       Impact factor: 16.971

10.  Error rates in oligodeoxynucleotides synthesized by the H-phosphonate method.

Authors:  M Vasser; P G Ng; P Jhurani; N Bischofberger
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971
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