Literature DB >> 1850122

Modified deoxyoligonucleotides stable to exonuclease degradation in serum.

J P Shaw1, K Kent, J Bird, J Fishback, B Froehler.   

Abstract

Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by simple terminal modifications that maintain high binding activity for the complementary DNA sequence.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1850122      PMCID： PMC333706          DOI： 10.1093/nar/19.4.747

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Relative stability of parallel- and antiparallel-stranded duplex DNA.

Authors:  M W Germann; B W Kalisch; J H van de Sande
Journal:  Biochemistry       Date:  1988-11-01       Impact factor: 3.162

2.  A quantitative bioassay for HIV-1 based on trans-activation.

Authors:  B K Felber; G N Pavlakis
Journal:  Science       Date:  1988-01-08       Impact factor: 47.728

3.  Regulation of viral expression of human immunodeficiency virus in vitro by an antisense phosphorothioate oligodeoxynucleotide against rev (art/trs) in chronically infected cells.

Authors:  M Matsukura; G Zon; K Shinozuka; M Robert-Guroff; T Shimada; C A Stein; H Mitsuya; F Wong-Staal; J S Cohen; S Broder
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

4.  Synthesis of DNA via deoxynucleoside H-phosphonate intermediates.

Authors:  B C Froehler; P G Ng; M D Matteucci
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971

5.  Phosphorothioate analogs of oligodeoxynucleotides: inhibitors of replication and cytopathic effects of human immunodeficiency virus.

Authors:  M Matsukura; K Shinozuka; G Zon; H Mitsuya; M Reitz; J S Cohen; S Broder
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

6.  Effects of pendant groups at phosphorus on binding properties of d-ApA analogues.

Authors:  R L Letsinger; S A Bach; J S Eadie
Journal:  Nucleic Acids Res       Date:  1986-04-25       Impact factor: 16.971

7.  Physicochemical properties of phosphorothioate oligodeoxynucleotides.

Authors:  C A Stein; C Subasinghe; K Shinozuka; J S Cohen
Journal:  Nucleic Acids Res       Date:  1988-04-25       Impact factor: 16.971

8.  Parallel stranded DNA.

Authors:  J H van de Sande; N B Ramsing; M W Germann; W Elhorst; B W Kalisch; E von Kitzing; R T Pon; R C Clegg; T M Jovin
Journal:  Science       Date:  1988-07-29       Impact factor: 47.728

9.  Phosphoramidate analogues of DNA: synthesis and thermal stability of heteroduplexes.

Authors:  B Froehler; P Ng; M Matteucci
Journal:  Nucleic Acids Res       Date:  1988-06-10       Impact factor: 16.971

10.  Partial protection of oncogene, anti-sense oligodeoxynucleotides against serum nuclease degradation using terminal methylphosphonate groups.

Authors:  D M Tidd; H M Warenius
Journal:  Br J Cancer       Date:  1989-09       Impact factor: 7.640
View more
  81 in total

1.  Modulation of human interferon-gamma biosynthesis by antisense oligodeoxynucleotides.

Authors:  C M Boeve; M De Ley
Journal:  Mol Biotechnol       Date:  2000-02       Impact factor: 2.695

2.  The stability of different forms of double-stranded decoy DNA in serum and nuclear extracts.

Authors:  B C Chu; L E Orgel
Journal:  Nucleic Acids Res       Date:  1992-11-11       Impact factor: 16.971

3.  Kinetics of DNA and RNA Hybridization in Serum and Serum-SDS.

Authors:  Elton Graugnard; Amber Cox; Jeunghoon Lee; Cheryl Jorcyk; Bernard Yurke; William L Hughes
Journal:  IEEE Trans Nanotechnol       Date:  2010-09-01       Impact factor: 2.570

4.  Circular Bivalent Aptamers Enable in Vivo Stability and Recognition.

Authors:  Hailan Kuai; Zilong Zhao; Liuting Mo; Hui Liu; Xiaoxiao Hu; Ting Fu; Xiaobing Zhang; Weihong Tan
Journal:  J Am Chem Soc       Date:  2017-06-29       Impact factor: 15.419

5.  Telomerase regulation of myofibroblast differentiation.

Authors:  Tianju Liu; Biao Hu; Myoung Ja Chung; Matt Ullenbruch; Hong Jin; Sem H Phan
Journal:  Am J Respir Cell Mol Biol       Date:  2006-01-19       Impact factor: 6.914

6.  Inhibition of human immunodeficiency virus type 1 Rev-Rev-response element complex formation by complementary oligonucleotides.

Authors:  D J Chin
Journal:  J Virol       Date:  1992-01       Impact factor: 5.103

Review 7.  The Role of RNA in Biological Phase Separations.

Authors:  Marta M Fay; Paul J Anderson
Journal:  J Mol Biol       Date:  2018-05-10       Impact factor: 5.469

8.  Stability and functional effectiveness of phosphorothioate modified duplex DNA and synthetic 'mini-genes'.

Authors:  S A Ciafrè; M Rinaldi; P Gasparini; D Seripa; L Bisceglia; L Zelante; M G Farace; V M Fazio
Journal:  Nucleic Acids Res       Date:  1995-10-25       Impact factor: 16.971

9.  Circulation of oligonucleotides by disulfide bridge formation.

Authors:  H Gao; M Yang; R Patel; A F Cook
Journal:  Nucleic Acids Res       Date:  1995-06-11       Impact factor: 16.971

10.  Ex vivo regulation of specific gene expression by nanomolar concentration of double-stranded dumbbell oligonucleotides.

Authors:  C Clusel; E Ugarte; N Enjolras; M Vasseur; M Blumenfeld
Journal:  Nucleic Acids Res       Date:  1993-07-25       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.