Literature DB >> 32684898

Hydrogen/deuterium exchange behavior in tetragonal hen egg-white lysozyme crystals affected by solution state.

Akiko Kita1, Yukio Morimoto1.   

Abstract

Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period. © International Union of Crystallography 2020.

Entities:  

Keywords:  H/D exchange; X-ray/neutron joint refinement; crystal structure; hen egg-white lysozyme

Year:  2020        PMID： 32684898      PMCID： PMC7312140          DOI： 10.1107/S1600576720005488

Source DB:  PubMed          Journal:  J Appl Crystallogr        ISSN： 0021-8898            Impact factor:   3.304


  22 in total

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9.  High-resolution structure (1.33 A) of a HEW lysozyme tetragonal crystal grown in the APCF apparatus. Data and structural comparison with a crystal grown under microgravity from SpaceHab-01 mission.

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  3 in total

1.  Hydrogen/Deuterium Exchange Behavior During Denaturing/Refolding Processes Determined in Tetragonal Hen Egg-White Lysozyme Crystals.

Authors:  Akiko Kita; Yukio Morimoto
Journal:  Mol Biotechnol       Date:  2022-01-13       Impact factor: 2.695

2.  Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme.

Authors:  Joao Ramos; Valerie Laux; Michael Haertlein; Elisabetta Boeri Erba; Katherine E McAuley; V Trevor Forsyth; Estelle Mossou; Sine Larsen; Annette E Langkilde
Journal:  IUCrJ       Date:  2021-03-06       Impact factor: 4.769

3.  The impact of folding modes and deuteration on the atomic resolution structure of hen egg-white lysozyme.

Authors:  Joao Ramos; Valerie Laux; Michael Haertlein; V Trevor Forsyth; Estelle Mossou; Sine Larsen; Annette E Langkilde
Journal:  Acta Crystallogr D Struct Biol       Date:  2021-11-17       Impact factor: 7.652
  3 in total

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