Literature DB >> 3178748

The electrostatic fields in the active-site clefts of actinidin and papain.

R W Pickersgill1, P W Goodenough, I G Sumner, M E Collins.   

Abstract

The active sites of actinidin (EC 3.4.22.14) and papain (EC 3.4.22.2) display different reactivity characteristics to probes targeted at the active-site cysteine residue despite the close structural similarity of their active sites. The calculated electrostatic fields in the active-site clefts of actinidin and papain differ significantly and may explain the reactivity characteristics of these enzymes. Calculation of electrostatic potential also focuses attention on the electrostatic properties that govern formation of the active-site thiolate-imidazolium ion-pair. These calculations will guide the modification of the pH-activity profile of the cysteine proteinases by site-directed mutagenesis.

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Year:  1988        PMID： 3178748      PMCID： PMC1135062          DOI： 10.1042/bj2540235

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  The Protein Data Bank: a computer-based archival file for macromolecular structures.

Authors:  F C Bernstein; T F Koetzle; G J Williams; E F Meyer; M D Brice; J R Rodgers; O Kennard; T Shimanouchi; M Tasumi
Journal:  J Mol Biol       Date:  1977-05-25       Impact factor: 5.469

2.  Electrostatic interactions in globular proteins: calculation of the pH dependence of the redox potential of cytochrome c551.

Authors:  N K Rogers; G R Moore; M J Sternberg
Journal:  J Mol Biol       Date:  1985-04-20       Impact factor: 5.469

3.  Anionic proteinase from Actinidia chinensis. Preparation and properties of the crystalline enzyme.

Authors:  M A McDowall
Journal:  Eur J Biochem       Date:  1970-06

4.  The interpretation of protein structures: estimation of static accessibility.

Authors:  B Lee; F M Richards
Journal:  J Mol Biol       Date:  1971-02-14       Impact factor: 5.469

5.  Calculation of the electric potential in the active site cleft due to alpha-helix dipoles.

Authors:  J Warwicker; H C Watson
Journal:  J Mol Biol       Date:  1982-06-05       Impact factor: 5.469

6.  Structure of actinidin, after refinement at 1.7 A resolution.

Authors:  E N Baker
Journal:  J Mol Biol       Date:  1980-08-25       Impact factor: 5.469

7.  Differences between the electric fields of the catalytic sites of papain and actinidin detected by using the thiol-located nitrobenzofurazan label as a spectroscopic reporter group.

Authors:  K Brocklehurst; E Salih; T S Lodwig
Journal:  Biochem J       Date:  1984-06-01       Impact factor: 3.857

8.  The alpha-helix dipole and the properties of proteins.

Authors:  W G Hol; P T van Duijnen; H J Berendsen
Journal:  Nature       Date:  1978-06-08       Impact factor: 49.962

9.  A marked gradation in active-centre properties in the cysteine proteinases revealed by neutral and anionic reactivity probes. Reactivity characteristics of the thiol groups of actinidin, ficin, papain and papaya peptidase A towards 4,4'-dipyridyl disulphide and 5,5'-dithiobis-(2-nitrobenzoate) dianion.

Authors:  K Brocklehurst; S M Mushiri; G Patel; F Willenbrock
Journal:  Biochem J       Date:  1983-03-01       Impact factor: 3.857

10.  pH-dependence and structure-activity relationships in the papain-catalysed hydrolysis of anilides.

Authors:  G Lowe; Y Yuthavong
Journal:  Biochem J       Date:  1971-08       Impact factor: 3.857
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  9 in total

1.  An interpretation of fluctuations in enzyme catalysis rate, spectral diffusion, and radiative component of lifetimes in terms of electric field fluctuations.

Authors:  Meher K Prakash; R A Marcus
Journal:  Proc Natl Acad Sci U S A       Date:  2007-10-02       Impact factor: 11.205

2.  Ionic strength independence of charge distributions in solvation of biomolecules.

Authors:  J J Virtanen; T R Sosnick; K F Freed
Journal:  J Chem Phys       Date:  2014-12-14       Impact factor: 3.488

3.  The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.

Authors:  D Kowlessur; M O'Driscoll; C M Topham; W Templeton; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

4.  The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain.

Authors:  K Brocklehurst; M O'Driscoll; D Kowlessur; I R Phillips; W Templeton; E W Thomas; C M Topham; C W Wharton
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

5.  Structural and electrostatic differences between actinidin and papain account for differences in activity.

Authors:  R W Pickersgill; I G Sumner; M E Collins; P W Goodenough
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

Review 6.  Rethinking peripheral T cell tolerance: checkpoints across a T cell's journey.

Authors:  Mohamed A ElTanbouly; Randolph J Noelle
Journal:  Nat Rev Immunol       Date:  2020-10-19       Impact factor: 53.106

7.  Ionic interactions near the loop L4 are important for maintaining the active-site environment and the dimer stability of (pro)caspase 3.

Authors:  Brett Feeney; Cristina Pop; Ashutosh Tripathy; A Clay Clark
Journal:  Biochem J       Date:  2004-12-15       Impact factor: 3.857

8.  Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group.

Authors:  G W Mellor; S K Sreedharan; D Kowlessur; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

9.  Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.

Authors:  M P Thomas; C M Topham; D Kowlessur; G W Mellor; E W Thomas; D Whitford; K Brocklehurst
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857
  9 in total

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